Changes of serum miRNA-21 and its correlation with apoptosis factors in acute myocardial infarction
Objective To analyze the changes of serum microRNA-21(miRNA-21)and its correlation with apoptosis factors[cysteine proteinase-3(caspase-3),soluble tumor necrosis factor-related apoptosis-inducing ligand(sTRAIL),soluble apoptosis-related factor(sFas)]in patients with acute myocardial infarction(AMI). Methods A total of 112 patients with AMI at Daxing District People's Hospital,Beijing,were enrolled as the study group between April 2020 and April 2022. Based on the severity of their coronary lesions,they were divided into three groups:single-vessel group(n=30),double-vessel group(n=48)and three-vessel group (n=34). A total of 70 healthy individuals during the same period were enrolled as the control group. The levels of serum miRNA-21 were measured using real-time fluorescence quantitative polymerase chain reaction. The levels of serum caspase-3,sTRAIL and sFas were measured using enzyme-linked immunosorbent assay. The levels of serum miRNA-21,caspase-3,sTRAIL and sFas were compared among all participants. The correlation between miRNA-21 and caspase-3,sTRAIL,and sFas was analyzed using Pearson coefficient. The diagnostic value of serum miRNA-21,caspase-3,sTRAIL and sFas for AMI was determined using ROC curves,and their correlation with the severity of coronary lesions was analyzed using the Spearman coefficient. Results The levels of serum miRNA-21,caspase-3,sTRAIL and sFas in the study group were higher than those in the control group(P<0.05). Pearson correlation analysis showed that serum miRNA-21 was positively correlated with caspase-3,sTRAIL and sFas(P<0.05). The ROC curve analysis showed that the AUC of miRNA-21 combined with Caspase-3,sTRAIL and sFas in the diagnosis of AMI was 0.940,which was greater than that of asingle indicator(0.788,0.749,0.775,0.756,P<0.05). The levels of serum miRNA-21,caspase-3, sTRAIL and sFas were the highest in the three-vessel group,followed by the double-vessel group and the single-vessel group(P<0.05). Spearman correlation analysis showed that miRNA-21,caspase-3,sTRAIL and sFas were positively correlated with the severity of coronary lesions(P<0.05). Conclusion The expressions of se-rum miRNA-21 and apoptosis factors are up regulated in patients with AMI. These expression levels are correlated with the severity of coronary lesions and are expected to serve as reference indicators for diagnosing AMI.
AMImiRNA-21Apoptosis factorSeverity of coronary lesion
万方数据
维普
