Relationship between circulating tumor cells in breast cancer and clinical features as well as their application in monitoring treatment efficacy
Objective To analyze the relationship between circulating tumor cells (CTCs) and the clinical features of breast cancer patients and explore their value in monitoring the efficacy of breast cancer treatment. Methods A total of 39 female breast cancer patients and 30 patients with benign breast diseases who attended Hefei Cancer Hospital of the Chinese Academy of Sciences between June 2021 and November 2022 were selected. The number of CTCs in the peripheral blood of patients was detected using negative enrichment and immunofluorescence in situ hybridization(imFISH). The correlation between CTCs and clinical features as well as the difference in CTC count before and after treatment were analyzed. Results The positive rates of CTCs in breast cancer patients before and after treatment were 87.2％and 84.6％,respectively. CTCs were not detected in patients with benign breast diseases. The positive rate of CTCs in TNM stage Ⅲ～Ⅳ patients was significantly higher than that in patients with stage Ⅰ～Ⅱ,and the difference was statistically significant(x2=4.848,P=0.047). The positive rate of CTCs in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis,and the difference was statistically significant(x2=10.469,P=0.007). After treatment,4 of the 39 patients showed disease progression,30 had stable disease, and 5 had partial remission. There was no significant difference in the number of CTCs before and after treatment in patients with disease progression(P>0.05). However,the number of CTCs after treatment in patients with stable and partial remission was significantly lower than before treatment ,and the difference was statistically significant(P<0.05). Conclusion The detection rate of CTCs using the negative enrichment and immunofluorescence in situ hybridization method is high. CTC detection is helpful in evaluating the condition of breast cancer patients and has good application value for monitoring the efficacy of breast cancer.
万方数据
维普
