The influence of previous factors on the detection results of 25-hydroxyvitamin D with different detection principles
Objective To investigate the impact of pre-analytical factors on the results of 25-hydroxyvitamin D detection using different detection. Methods The serum and plasma samples from inpatients at Beijing Aerospace General Hospital were collected as research objects. Immunoassay and liquid chromatography tandem mass spectrometry were used to detect 25-hydroxyvitamin D respectively. The effects of four fac-tors on the detection results of the two methods were analyzed:the centrifugation temperature was room temperature and 4℃,anticoagulant tube and procoagulant tube,centrifugation immediately after collection and after storage at room temperature for 8 hours,storage at room temperature and 2-8℃for 8 hours after centrifugation. Results The immunoassay method was used to detect 25(OH)D levels. The results showed that the room temperature centrifugation group,blood collection group with procoagulant tube and the group where blood was left at room temperature for 8 hours had higher levels compared to the 4℃ centrifugation group,8 anticoagulant tube group,and immediate centrifugation group. These differences were statistically significant (t=-7.398,2.852,3.506,P<0.05). Additionally,the results of the group stored at room temperature were higher than those stored at 2-8℃,but this difference was not statistically significant(t=1.883,P>0.05). Mass spectrometry was used to detect 25(OH)D levels. Similar to the immunoassay results,the levels in the room temperature centrifugation group,blood collection group with with a procoagulant tube,group where blood was left at room temperature for 8 hours,and the storage group at room temperature after centrifugation were higher than those in the 4℃ centrifugation group,anticoagulant tube group,immediate centrifugation group, and storage group at 2～8℃. However,there were no significant differences found(t=-1.918,-1.995,-1.499,-0.399,P>0.05). Conclusion The influence of pre-factors on the 25-hydroxyvitamin D test results of two different test principles varies. It is important to implement effective measures to prevent pre-factors from affecting the accuracy of test results during daily testing.
[25(OH)D]Liquid chromatography tandem mass spectrometryImmunoassay
万方数据
维普
