Value of combined detection of serum miR-31 and IL-22 in diagnosis and prognosis assessment of viral myocarditis in children
Objective To study the value of serum microRNA-31(miR-31)and interleukin-22 (IL-22)in the diagnosis and prognosis of viral myocarditis in children. Methods Children with viral myocarditis(VMC)who were admitted to the Cardiovascular Department of Henan Children's Hospital from January 2017 to January 2022 were selected as the study subjects and referred to as the VMC group(n=106). Healthy children who underwent physical examinations in the hospital during the same period were selected as the control group(n=70). Serum levels of miR-31,IL-22,creatine kinase isoenzyme(CK-MB),cardiac troponin T (cTnT),ECG parameters [QRS interval,PR interval] and echocardiographic parameters [left ventricular ejection fraction(LVEF),left ventricular short axis shortening rate(LVFS)]were compared between the two groups. Patients with VMC were followed up for 1 year after the completion of treatment. Poor prognosis events such as delayed recovery,dilated cardiomyopathy,residual arrhythmia and child death were considered durinh the follow-up period. The influencing factors of poor prognosis in children with VMC were analyzed using multivariate logistic regression. The ROC curve was drawn to analyze the value of serum miR-31 and IL-22 levels in diagnosis and poor prognosis assessment of children with VMC. Results Serum levels of miR-31, IL-22,CK-MB,cTnT,QRS interval and PR interval in the VMC group were higher than those in the control group,with statistical significance(P<0.05). LVEF and LVFS were lower than the control group,and the difference was statistically significant (P<0.05). Multivariate logistic regression analysis showed that high expression of miR-31,elevated IL-22,elevated CK-MB,elevated cTnT,prolonged QRS interval,prolonged PR interval,decreased LVEF and decreased LVFS were independent risk factors for poor prognosis in children with VMC(P<0.05). The ROC curve analysis showed that the area under the curve(AUC)of the combined detection of serum miR-31 and IL-22 levels was 0.990,which was better than that of single detection(P<0.05). The area under the curve(AUC)of the combined detection of serum miR-31 and IL-22 was 0.919, which was better than that of single detection(P<0.05). Conclusion miR-31 and IL-22 are highly expressed in the serum of children with viral myocarditis. This finding suggests that they could serve as auxiliary diagnostic indicator for the diagnosis and prognosis evaluation of viral myocarditis in children.
万方数据
维普
