Application value of anti-desmoglein 1 and 3 antibodies and bullous pemphigoid 180 and 230 antibodies in the diagnosis of bullous dermatosis
Objective To explore the application value in the clinical diagnosis of bullous derma-tosis by detecting antibodies against desmoglein 1,3(DSG 1,3)and bullous pemphigoid 180,230(BP 180, 230). Methods This study was a retrospective,double-blind trial conducted from March 2020 to January 2023. The study included 88 patients with Bullous pemphigoid diagnosed based on histopathological results and direct immunofluorescence detection at our hospital as the observation group. Additionally ,76 patients who un-derwent physical examinations at our hospital during the same period were selected as the control group. The study aims to analyze the positive detection of Dsg1,Dsg3,BP180,and BP230 antibodies in the serum of all study subjects. It also aimed to determine the percentage of diagnostic types of serum Dsg1,Dsg3,BP180, and BP230 antibody specimens in the observation group,as well as the percentage of positive rates of Bullous pemphigoid disease types in different age groups within the observation group. Furthermore ,the study aimed to draw the ROC curves to analyze the diagnostic value of single and combined testing of Dsg1,Dsg3, BP180,and BP230 antibodies for Bullous pemphigoid. Results The difference in the positive rates of serum Dsg1,Dsg3,BP180 and BP230 antibodies between the two groups was statistically significant(P<0.05). Ac-cording to the results of the Dsg1,Dsg3,BP180 and BP230 antibody tests,the positive rates of diagnostic types for BP,PV,PF,PF+BP and PV+BP were 32.95％,20.45％,11.36％,7.95％and 3.41％,respective-ly. The percentage of BP positivity was the highest,while the percentage of PV+BP positivity was the lowest, and the difference between them was statistically significant(P<0.05). The rate of BP positivity was higher in the>60-year-old group than in the≤ 60-year-old group,and the rate of PV and PF positivity was lower than in the≤ 60-year-old group(P<0.05). The sensitivity and specificity of the combined detection of antibodies against Dsg1,Dsg3,BP180 and BP230 were 91.02％ and 88.66％ respectively,and the AUC (95％CI) was 0.821 (0.745～0.911),which was higher than the single detection of the above antibodies (P<0.05). Conclusion Dsg1,Dsg3,BP180,and BP230 antibodies have significant reference value in the diagnosis of Bullous pemphi-goid. The combination of these tests can improve diagnostic accuracy. There is also a correlation between the age of patients with Bullous pemphigoid and the type of antibody. Combined detection of Dsg1,Dsg3,BP180,and BP230 antibodies is helpful for a more precise diagnosis and treatment of Bullous skin diseases.
Bridging core protein 1Bridging core protein 3Herpetic pemphigoid 180Herpetic pemphigoid 230AntibodyBullous pemphigoid
万方数据
维普
