Objective To explore the expression and key target gene prediction of enhancer RNA RASSF8-AS1 in stomach adenocarcinoma(STAD),and to analyze the relationship between RASSF8-AS1 and clinicopathological features and prognosis,as well as explore the mechanism of RASSF8-AS1 in the occurrence and development of STAD. Methods Expression data,survival data,and clinical data for 33 tumor types from the UCSC Xena database were download. Kaplan-Meier survival analysis and correlation analysis were used to identify key eRNAs and their regulatory genes as eRNA-target gene pairs. The ggboxplot command of the R language was used to analyze the correlation between RASSF8-AS1 expression and patient clinicopatholo-gy. Additionally,GO and KEGG enrichment analysis were used to explore the signaling pathways involved in RASSF8-AS1 in STAD. Reverse transcription polymerase chain reaction(RT-qPCR)was used to validate the expression of RASSF8-AS1 in normal and gastric adenocarcinoma cell lines. Results The expression level of RASSF8 was significantly correlated with the patient ' s age,clinical grade,and clinical stage (c2=4.356,4.166,6.452,P<0.05). RT⁃qPCR results showed that compared with normal cells,the expression levels of RASSF8 ⁃ AS1 and RASSF8 in gastric cancer cells were significantly decreased,with a statistically significant difference(HR=0.044,95％CI:0.032～0.056,P<0.05). The overall survival rate of patients in the high ex⁃pression group of RASSF8⁃AS1 was significantly lower than that of the patients in the low expression group,with a statistically significant difference(P<0.05). GO analysis showed that RASSF8⁃AS1 was involved in vari⁃ous biological processes such as extracellular matrix organization,cell adhesion,regulation of chemical synap⁃tic transmission,and collagen metabolism. In the KEGG pathway analysis,signaling pathways such as intersti⁃tial development,extracellular matrix organization,and regulation of membrane potential were enriched.Conclusion RASSF8⁃AS1 is a crucial survival⁃related eRNA in gastric cancer and could serve a promising bio⁃marker and therapeutic target for the early diagnosis of patients with gastric cancer.
维普
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