Correlation and clinical significance of miR-145,Fascin-1 and OCT4 in infertile patients with endometriosis
Objective To investigate the correlation between miR-145 and Fascin-1,OCT4 of in-fertile patients with endometriosis(EMs)and its clinical significance. Methods A total of 100 infertility pa-tients with EMs who underwent laparoscopic surgery at the First People ' s Hospital of Chongqing High-tech Zone from January 2020 to January 2022 were selected for the EMs group,and ectopic endometrial tissues were collected. Additionally,55 patients who underwent hysteroscopic surgery for intrauterine adhesions were chosen for the control group,and normal endometrial tissues were collected. The expression levels of miR-145, Fascin-1,and OCT4 in the endometrial tissues of both groups as well as in patients with different AFS stages in the EMs group were measured. The correlation between miR-145 and Fascin-1,OCT4 expression in the EMs group was analyzed. The spontaneous pregnancy rates of infertility patients who underwent EMs surgery were followed up for the first year post-surgery,and the rates were compared based on different miR-145 ex-pression levels in the Ems group. Results The expression levels of miR-145 and the mRNA and protein ex-pression levels of Fascin-1 and OCT4 in endometrial tissues were significantly higher in the Ems group com-pared to the control group(P<0.05). Within the EMs group,the expression levels of miR-145 and the mRNA and protein expression levels of Fascin-1 and OCT4 in endometrial tissues were higher in patients with AFS Ⅲ-Ⅳcompared to those with AFS Ⅰ-Ⅱ,and these differences were statistically significant(P<0.05). The expression level of miR-145 in endometrial tissues of the EMs group was positively correlated with the mRNA and protein expression of Fascin-1 and OCT4(P<0.05). Based on the median expression level of miR-145 in endometrial tissues of patients in the EMs group,the patients were divided into two groups:the high expression and low expression. The cumulative postoperative spontaneous pregnancy rate was significantly lower in patients with high miR-145 expression compared to those with low miR-145 expression (P<0.05). Conclusion The in-crease in miR-145 expression in endometrial tissue with endometriosis-related infertility is associated with in-creased expression of Fascin-1 and Oct4,as well as a decrease in the natural pregnancy rate.
万方数据
维普
