Changes in serum HSP 70 levels and analysis of HSP 70-1 gene polymorphisms in 128 patients with viral infection
Objective To investigate the changes in serum HSP 70 expression and its relationship with HSP 70-1 gene polymorphism in 128 patients with novel coronavirus infection at a hospital in Guizhou Province. Methods A total of 128 patients with novel coronavirus infection (case group) and 128 healthy subjects(control group)who were admitted to a hospital in Guizhou Province from January 2020 to February 2020 were selected for the study. The changes in serum HSP 70 levels between the two groups were compared using enzyme-linked immunosorbent assay(ELISA). The single nucleotide polymorphism(SNP)genotyping method was used to detect the HSP70-1 gene sequence at the + 190 locus and analyze gene polymorphism. Results The serum HSP 70 protein levels in the case group were higher than those in the control group ,with a statistically significant difference (Z=6.924,P<0.05). Additionally,the serum HSP 70 levels in the case group increased gradually as the disease progressed. The HSP 70 levels in each clinical classification were high-er than those in the control group,and the differences were statistically significant(P<0.05). In the patient case group,HSP 70-1(+190 locus)genotypes GG,GC and CC were identified,with a statistically signifi-cant difference compared to the control group(c2=6.975,P<0.05). In addition,the distribution frequency of the HSP 70-1 GC genotype in mild patients(59.26％)and common patients(52.69％)was higher than that in the control group(39.84％)(P<0.05). Conversely,the distribution frequency of the HSP 70-1 GG genotype in mild patients (33.33％) and common patients (38.71％) was lower than that in the control group (53.90％),with a statistically significant difference(P<0.05). Conclusion The expression levels of HSP 70 and the HSP 70-1 genotype are related to the occurrence and development of novel coronavirus infection. This can help in determining the clinical classification of patients with novel coronavirus infection.
万方数据
维普
