摘要
目的 分析贵州省某院128例新型冠状病毒感染患者血清HSP 70表达水平及HSP 70-1基因多态性变化,并探讨其临床价值.方法 选取2020年1月至2020年2月贵州省某医院收治的128例新型冠状病毒感染患者(病例组),同期128名健康体检者(对照组).采用酶联免疫吸附试验(ELISA)比较两组血清中HSP 70水平变化;采用单核苷酸多态性(SNP)基因分型方法检测HSP 70-1(+190位点)基因序列,分析基因多态性.结果 病例组患者血清中HSP 70蛋白高于对照组,差异有统计学意义(Z=6.924,P<0.05),并且随着病例组患者病程的加重,血清HSP 70水平也逐渐升高,各临床分型的HSP 70水平均高于对照组,差异均有统计学意义(P<0.05);HSP 70-1(+190位点)的基因型GG、GC、CC在患者病例组均存在,与对照组比较,差异有统计学意义(c2=6.975,P<0.05),此外,轻型患者HSP 70-1 GC基因型的分布频率(59.26％)及普通型患者HSP 70-1 GC基因型的分布频率(52.69％)均高于对照组(39.84％)(P<0.05),而轻型患者HSP 70-1 GG基因型的分布频率(33.33％)及普通型患者HSP 70-1 GG基因型的分布频率(38.71％)均低于对照组(53.90％),差异有统计学意义(P<0.05).结论 HSP 70表达水平及HSP 70-1基因分型与新型冠状病毒感染的发生、发展相关,可辅助新型冠状病毒感染患者临床分型的判断.
Abstract
Objective To investigate the changes in serum HSP 70 expression and its relationship with HSP 70-1 gene polymorphism in 128 patients with novel coronavirus infection at a hospital in Guizhou Province. Methods A total of 128 patients with novel coronavirus infection (case group) and 128 healthy subjects(control group)who were admitted to a hospital in Guizhou Province from January 2020 to February 2020 were selected for the study. The changes in serum HSP 70 levels between the two groups were compared using enzyme-linked immunosorbent assay(ELISA). The single nucleotide polymorphism(SNP)genotyping method was used to detect the HSP70-1 gene sequence at the + 190 locus and analyze gene polymorphism. Results The serum HSP 70 protein levels in the case group were higher than those in the control group ,with a statistically significant difference (Z=6.924,P<0.05). Additionally,the serum HSP 70 levels in the case group increased gradually as the disease progressed. The HSP 70 levels in each clinical classification were high-er than those in the control group,and the differences were statistically significant(P<0.05). In the patient case group,HSP 70-1(+190 locus)genotypes GG,GC and CC were identified,with a statistically signifi-cant difference compared to the control group(c2=6.975,P<0.05). In addition,the distribution frequency of the HSP 70-1 GC genotype in mild patients(59.26％)and common patients(52.69％)was higher than that in the control group(39.84％)(P<0.05). Conversely,the distribution frequency of the HSP 70-1 GG genotype in mild patients (33.33％) and common patients (38.71％) was lower than that in the control group (53.90％),with a statistically significant difference(P<0.05). Conclusion The expression levels of HSP 70 and the HSP 70-1 genotype are related to the occurrence and development of novel coronavirus infection. This can help in determining the clinical classification of patients with novel coronavirus infection.
基金项目
贵州省卫生健康委科学技术基金(gzwjkj2020-1-062)
维普
万方数据