Correlation between PI3K-AKT/mTOR signaling pathway and Treg/Th17 immune balance in children with primary nephrotic syndrome
Objective To study the correlation between phosphatidylinositol 3- kinase (PI3K)-protein kinase B(AKT)/mammalian target protein of rapamycin(mTOR)and the immune balance of regulato-ry T cells(Treg)/ helper T cells(Th17)in children with primary nephrotic syndrome(PNS). Methods 30 children with PNS were admitted to Guigang People's Hospital from June 2020 to July 2022 and were selected as the nephropathy group. Additionally,30 healthy children who underwent physical examinations at the same hospital during the same period were chosen as the healthy group. The expression levels of PI3K,mTOR and Akt messenger ribonucleic acid(mRNA)in CD4+T cells and the levels of Treg,Th17 and Treg/Th17 in periph-eral blood were compared between the two groups,and the correlation between the expression levels of PI3K, mTOR and Akt mRNA in CD4+T cells in children with PNS and the levels of Treg,Th17 and Treg/Th17 in pe-ripheral blood was analyzed. Results The expression levels of PI3K,mTOR,and Akt mRNA in CD4+T cells of the nephropathy group and Th17 in the peripheral blood were higher than those in the healthy group. These differences were statistically significant (t=10.694,3.038,8.915,7.148,P<0.05). The levels of Treg and Treg/Th17 in the peripheral blood of the nephropathy group were lower than those in the healthy group ,with a statistically significant difference (t=8.269,15.780,P<0.05). The expression levels of PI3K,mTOR,and Akt mRNA in peripheral blood CD4+T cells of children with PNS were significantly negatively correlated with the levels of Treg and Treg/Th17 in the peripheral blood(r=-0.637,-0.573,-0.492,-0.559,-0.511,-0.612, P<0.05),and significantly positively correlated with Th17(r=0.479,0.495,0.603,P<0.05). Conclusion The occurrence of PNS could lead to abnormal expression levels of the PI3K-AKT/mTOR signaling pathway in children's peripheral blood CD4+T cells. Simultaneously,it could also cause an imbalance between Treg and Th17 immune cells the body. There was a clear correlation between the PI3K-AKT/mTOR signaling pathway in children's peripheral blood CD4+T cells and the Treg/Th17 immune balance.
Primary nephrotic syndromePhosphatidylinositol 3-kinaseProtein kinase bMammalian rapamycin target proteinSignal pathwayRegulatory T cellsHelper T cellsImmune balance
万方数据
维普
