Relationship between MAPK pathway in peripheral blood mononuclear cells and inflammatory response,no reflow after PCI in patients with STEMI
Objective To investigate the relationship between mitogen-activated protein kinase (MAPK)pathway in peripheral blood mononuclear cells(PBMCs)and inflammatory response,no reflow af-ter percutaneous coronary intervention(PCI)in patients with STEMI. Methods STEMI patients who under-went PCI from February 2021 to October 2022 and healthy volunteers who underwent physical examinations during the same period were divided into the STEMI group(n=92)and the control group(n=100),respec-tively. The expression levels of p38MAPK and JNK in PBMCs were detected using fluorescence quantitative PCR. Based on the median,the patients in the STEMI group were divided into high expression and low expres-sion groups for p38MAPK and JNK. The levels of ICAM-1,TNF-α and IL-6 in serum were tested,and the degree of myocardial injury and no reflow in STEMI patients were evaluated. Result The expression levels of p38MAPK and JNK in PBMCs of the STEMI group were higher than those of the control group ,and the differ-ence was statistically significant(t=5.386,5.126,P<0.05). The peak values of CK-MB,cTnT and the con-tents of ICAM-1,TNF-αand IL-6 in the serum of patients with high expression of p38MAPK and JNK in the STEMI group were higher than those of patients with low expression of p38MAPK and JNK. The difference was statistically significant(t=7.143,4.999,9.528,6.805,6.890,6.226,8.132,7.807,7.162,7.588,P<0.05). The expression levels of p38MAPK and JNK in PBMCs of patients with no reflow in the STEMI group were higher than those of patients with normal blood flow. The difference was statistically significant (t=5.208,4.240,P<0.05). The ROC curve analysis showed that the expression levels of p38MAPK and JNK in PBMCs can predict no reflow in STEMI patients after PCI. Conclusion The overexpression of p38MAPK and JNK in the MAPK pathway of STEMI patients is associated with the activation of inflammatory response and the occurrence of no reflow after PCI.
ST segment elevation myocardial infarctionPercutaneous coronary interventionMitogen activated protein kinaseInflammatory responseNo reflow
万方数据
维普
