Study on the mechanism of Mfn2 regulating VEGFR2/PI3K to promote ovarian cancer implantation and metastasis
Objective To investigate the role of the mitochondrial fusion protein 2 (Mfn2) in ovarian cancer implantation and metastasis and to explore its potential molecular mechanism. Methods The study selected 86 patients with ovarian cancer who were treated at Suzhou Hospital Affiliated with Nanjing Medical University from June 2019 to June 2021 as the study subjects. The protein expressions of Mfn2,vascu-lar endothelial growth factor receptor 2(VEGFR2)and phosphoinositide 3-kinase(PI3K)in cancer tissue and adjacent tissue were compared to analyze the relationship between Mfn2,VEGFR2 and PI3K protein expres-sion and related pathological features. An up-regulated/down-regulated SKOV-3 cell line was constructed to verify the expression of Mfn2,VEGFR2 and PI3K. Results The positive expression rates of Mfn2,VEGFR2 and PI3K in tumor tissues were higher than those in para-cancer tissues (c2 values were 4.597,6.456 and 3.930,with P values of 0.032,0.011 and 0.047,respectively). There were statistically significant differences in the expression of Mfn2,VEGFR2 and PI3K in ovarian tissues at different FIGO stages,lymph node metas-tasis,distant metastasis and survival conditions(P<0.05). Compared with the NC group,the relative expres-sion of Mfn2 mRNA and Mfn2,VEGFR2 and PI3K proteins in SKOV-3 cells of ovarian cancer in the Mfn2 up-regulated group were significantly up-regulated(P<0.05). Compared with the shNC group,the relative expres-sion of Mfn2 mRNA and Mfn2,VEGFR2,and PI3K proteins in SKOV-3 cells of ovarian cancer in the down-regulated shMfn2 group were significantly down-regulated(P<0.05). Conclusion Down-regulating Mfn2 ex-pression and VEGFR2 and PI3K expression levels can prevent ovarian cancer implantation and metastasis.
万方数据
维普
