Role of enhancer RNA FGB in liver metastasis of colorectal cancer
Objective To study the regulatory role of enhancer RNA(eRNAs)in liver metastasis of colorectal cancer,and further clarify the role of enhancer RNA FGB in liver metastasis of colorectal cancer.Methods The gene expression profiles of GSE178120 in 105 cases of primary colorectal cancer and GSE15921 in 283 cases of liver metastasis of colorectal cancer were downloaded from the National Center for Biotechnology Information(NCBI)database.WGCNA analysis,Lasso regression analysis,multiple Cox re-gression analysis,Pearson correlation analysis and survival analysis were used to identify the core eRNA genes in colorectal cancer liver metastasis.The effects of the eRNA gene(FGB)on cell proliferation,migration and invasiveness were studied using CCK8,clonal formation and transwell methods.Results Bioinformatics analy-sis identified two eRNAs(FGB and SLC38A4)with significant prognostic value(P<0.05),where FGB(P=0.00423)showed greater significance than SLC38A4(P=0.01478).Results of in vitro cell experiments demon-strated that overexpression of FGB could suppress the proliferation and clonogenesis of colorectal cancer cell lines,as well as inhibit the metastasis of colorectal cancer cells.Conclusions eRNA plays an important role in the regulatory mechanism of liver metastasis in colorectal cancer.Additionally,FGB is involved in the molecu-lar regulation of liver metastasis in colorectal cancer.These findings provide a new research strategy for the early diagnosis and treatment of liver metastasis in colorectal cancer.
Colorectal cancerLiver metastasis of colorectal cancerPrediction modeleRNAFGB
NETL
NSTL
万方数据
