Comparison of the effectiveness of nanomagnetic beads modified with different bioactive groups in nucleic acid extraction
Objective To evaluate the nucleic acid extraction efficiency of nanomagnetic beads modified with different bioactive groups to provide some basis for clinical application and outcome evaluation.Methods Nucleic acid was extracted from samples of the hepatitis B virus using nano-magnetic beads modi-fied with different bioactive groups.The concentration and purity of the nucleic acid were measureed using NanoDrop Onec,and the CT values of the samples were determined using real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).Results Among several nanomagnetic bead extraction methods,the highest nucleic acid concentration and purity are achieved by superparamagnetic carboxyl nanoparticles(with an average concentration of 953.3 IU/mL).This is followed by silica magnetic microspheres,carboxyl micro-spheres mixed nanomagnetic beads,and the nano-magnetic oxide MnFe2O4 extraction method.The nucleic ac-id concentration extracted by hydroxyl-modified silica particles and chitosan-coated magnetic beads is higher,but the purity is lower(with A260/280 and A260/230 ratios exceeding the standard range).For the same sam-ple,the CT value of nucleic acid amplification extracted by superparamagnetic carboxyl nanoparticles is(21.28±0.36),indicating significantly higher nucleic acid extraction efficiency compared to spin column extraction,with statistical significance.The nucleic acid extraction efficiency of silica magnetic microspheres,carboxyl mi-crospheres mixed nano-magnetic beads,hydroxyl-modified silica nano-magnetic beads,and chitosan-coated na-no-magnetic beads are all lower than that of the spin column extraction method.In addition,the extraction time of superparamagnetic carboxyl nanoparticles can be shortened to 15 minutes.Conclusion Superparamagnetic carboxyl nanoparticles have the highest efficiency in nucleic acid extraction,as well as precision and purity in nucleic acid products.They also hold great clinical application value.
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