Analysis of the relationship between P53,P16 and Ki-67 and recurrence after ESD operation in early esophageal cancer patients
Objective To investigate the relationship between suppressor protein gene P53(P53),suppressor protein gene P16(P16)and cell proliferating nuclear antigen Ki-67(Ki-67)and recurrence after endoscopic submucosal dissection(ESD)in patients with early esophageal cancer.Methods 80 patients with early esophageal cancer were admitted to Sanmenxia Central Hospital from January 2019 to January 2023 and were retrospectively selected as the study subjects.All patients underwent ESD treatment.The expression of P53,P16 and Ki-67 proteins was compared between cancer tissue and paracancer tissue(defined as tissue more than 3 cm from the tumor margin,with no tumor tissue or atypical hyperplasia observed under the micro-scope)in all patients.Patients were followed up for 1 year after the ESD operation and were divided into two groups:the recurrence group(n=20)and the non-recurrence group(n=60)based on whether esophageal can-cer recurred after the operation.The expression of P53,P16 and Ki-67 in the two groups was compared.Multi-variate logistic regression was used to analyze the influencing factors of ESD postoperative recurrence in early esophageal cancer patients.ROC curves were drawn to analyze the expression of P53,P16 and Ki-67 proteins and ESD postoperative recurrence in early esophageal cancer patients.Results Compared to adjacent tissues,the expression of P53 and Ki-67 proteins in cancer tissues was increased,while the expression of P16 protein was decreased(t=9.276,13.987,10.595,P<0.05).The expression of P53 and Ki-67 protein in the recur-rence group was higher than that in the non-recurrence group,and the expression of P16 protein was lower than that in the non-recurrence group(t=5.086,4.648,5.139,P<0.05).Multivariate logistic regression analy-sis showed that low tumor differentiation(OR=1.870),tumor invasion of submucosa(OR=1.808),lymph node metastasis(OR=2.089),high expression of P53 protein(OR=2.046),low expression of P16 protein(OR=1.988),and high expression of Ki-67 protein(OR=1.761)were aa independent risk factors for recur-rence in early esophageal cancer patients after surgery(P<0.05).ROC curve analysis showed that the expres-sion of P53,P16 and Ki-67 proteins,along with the AUC of combined detection were 0.803,0.828,0.834,and 0.942,respectively.Combined detection was found to be superior to single detection(P<0.05).Conclu-sion P53,P16 and Ki-67 proteins are related to the recurrence of early esophageal cancer in patients follow-ing ESD surgery.They can serve as auxiliary diagnostic indicators for predicting recurrence.
Early esophageal cancerEndoscopic submucosal dissectionSuppressor protein gene P53Suppressor protein gene P16Cell proliferation nuclear antigen Ki-67
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