Effects of hydrogen gas inhalation on hyperoxia-induced bronchopulmonary dysplasia in neonatal SD rats
Objective To investigate the effect of hydrogen inhalation on bronchopulmonary dys-plasia(BPD)in hyperoxy-induced neonatal SD rats.Methods The bronchopulmonary dysplasia model was established by exposing neonatal SD rats to of 85%hyperoxygen through inhalation.The rats were divided into foue groups:the control group(normal air)9,the BPD group(85%O2)13,the H2 group(2%H2)12 and the treat group(85%O2+2%H2)16.The body weight of each group was recorded.After 14 days,the rats were sacrificed,and blood and lung tissues were collected.Pathological sections were observed and evaluated using hematoxylin-eosin(HE)staining.The supernatant of lung tissue homogenate was used to detect the lung injury marker ceramide(Cer).Blood samples were taken to detect the brain injury marker tropont-binding pro-tein(S100B),and the myocardial injury marker myocardial troponin(cTnI).The results were then compared among the groups.Results The body weight increase in the BPD group was significantly slower than that in the control group(F=7.122,P<0.05).HE staining results showed that hyperoxia simplified the alveoli and sig-nificantly reduced the number of alveoli,which could be effectively improved by inhaling hydrogen gas.The average intercept of alveoli in the BPD group was greater than that in the treat group and the control group,and this difference was significantly different from the H2 group(F=24.951,P<0.05).The Cer level in the BPD group was higher than that in the control group,the H2 group and the treat group.The cTnI level in the BPD group was higher than that in the H2 group,the treat group and the control group.The S100B level in the BPD group was higher than that in the treat group,the H2 group,and the control group.These differences were statistically significant(F=19.361,26.321,13.235,P<0.05).Conclusion Inhaling H2 can improve lung injury,brain injury and myocardial injury.The mechanism may be related to antioxidants.
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