The expression levels of CCNA1 and CDC20 in colorectal cancer tissues and their clinical prognostic significance
Objective To investigate the expression levels of cyclin A1(CCNA1)and cyclin D20(CDC20)in colorectal cancer(CRC)tissues and their relationship with clinical manifestations and prognosis of patients.Methods A total of 146 CRC patients were selected.The cancer tissue specimens obtained from surgical treatment were included in the CRC group(n=146)and the corresponding adjacent tissues(>3 cm from the cancer tissue)were included in the adjacent group(n=146).The expression of CCNA1 and CDC20 was detected by immunohistochemistry.The relationship between the CCNA1,CDC20 and clinicopathological features of CRC patients was analyzed by χ2 test,and the related factors for the prognosis of CRC patients were explored by multivariate Cox regression analysis.Results The positive expression rates of CCNA1 and CDC20 in the CRC group were higher than those in the adjacent group,and the difference was statistically sig-nificant(P<0.05).The positive expression rates of CCNA1 and CDC20 in patients with poorly differentiated degree,TNM stage Ⅲ,and nerve invasion were higher than those in patients with moderately/well differentiat-ed degrees,TNM stage Ⅰ～Ⅱ,and no nerve invasion,and the difference was statistically significant(P<0.05).The 3-year survival rate of patients with medium/high differentiation,TNM stage Ⅰ to Ⅱ,no nerve in-vasion,CCNA1 negative and CDC20 negative was higher than that of patients with low differentiation,TNM stage Ⅲ,nerve invasion,CCNA1 positive and CDC20 positive,respectively,and the difference was statisti-cally significant(P<0.05).Poorly differentiated CRC(HR=2.125,95%CI:1.406～3.214),TNM stage Ⅲ(HR=2.614,95%CI:1.504～4.544),nerve invasion(HR=2.337,95%CI:1.498～3.647),CCNA1 positive(HR=2.765,95%CI:1.758～4.348),CDC20 positive(HR=3.550,95%CI:2.075～6.074)were the prognostic factors of CRC patients(P<0.05).Conclusion CCNA1 and CDC20 are both highly expressed in CRC tis-sues and are closely associated with differentiation,TNM stage,and nerve invasion.They are expected to serve as potential markers to aid in the prognosis of CRC patients.
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