Consistency assessment between iCubate-iC blood culture rapid detection system and traditional blood culture identification of drug susceptibility
Objective To evaluate consistency of blood culture traditional identification and anti-microbial susceptibility with a PCR amplification/fluorescence-labeled probe capture-based system,the iCu-bate-iC assay,for rapid detection of blood culture positive specimens.Methods A total of 121 clinical blood culture positive specimens from November 26,2017 to April 18,2018 were enrolled in this study.The consis-tency of the identification and resistance marker detection of the iCubate-iC system was evaluated against clini-cal routine culture identification and in vitro antimicrobial susceptibility results.Results The identification of 95.9％(116/121)samples using iCubate-iC assay agreed with the results of MALDI-TOF MS.There were four false negative results,all of which showed"not detected"for the corresponding iCubate-iC target probes.It is worth noting that a specimen containing both Staphylococcus aureus and S.epidermidis was correctly detected by the iCubate-iC assay.In composite samples of Escherichia coli and Pseudomonas aeruginosa,was not de-tected Among 31 staphylococci isolates,18 were positive for mecA by iC-GPC.However,one S.epidermidis isolate was susceptible to cefoxitin in vitro.Only one enterococci isolate was positive for vanA,but it was sus-ceptible to vancomycin in vitro.Results for detecting carbapenemase-encoding genes(such as KPC and NDM)were consistent with in vitro antimicrobial susceptibility testing.The consistency between ESBL gene(CTX-M group1)and β lactam drugs in vitro was unsatisfactory,but the results for detecting carbapenemase-encoding genes(KPC and NDM)were consistent with in vitro antimicrobial susceptibility testing.Conclusion The iCu-bate-iC system is reliable for detecting common bloodstream infection bacteria in clinical settings.It offers the advantages of being rapid and simple,making it a powerful tool for quickly diagnosing bloodstream infections.
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