基于CRISPR-Cas系统的分子诊断应用研究进展
Research progress on molecular diagnostic applications based on CRISPR-Cas system
黄佳 1房师松2
作者信息
- 1. 广东药科大学公共卫生学院,广东,广州 510006
- 2. 深圳市疾病预防控制中心,广东,深圳 518055
- 折叠
摘要
CRISPR-Cas系统是细菌抵御噬菌体入侵的适应性免疫系统,按照其效应蛋白的不同分为两大类.其在特异性识别切割目标序列的基础上,还附带有切割周围单链DNA和RNA分子的反式切割特性,这种特异的识别机制为分子诊断带来了新机会,本文简单介绍了CRISPR-Cas的机制和类型,重点关注已经用于分子诊断的CRISPR-Cas系统,列举了其应用和相关研究进展.
Abstract
The CRISPR/Cas system is an adaptive immune system used by bacteria to defend against phage invasion.It is divided into two categories according to its effector proteins.In addition to specifically recognizing and cutting target sequences,it also has the property of trans-cutting surrounding single-stranded DNA and RNA molecules.This specific recognition mechanism has brought new opportunities for molecular diagnosis.This article briefly introduces the mechanism and types of CRISPR-Cas,focusing on the CRISPR-Cas system that has been used for molecular diagnosis,and lists its applications and related research progress.
关键词
CRISPR-Cas/Cas蛋白/分子诊断Key words
CRISPR-Cas/Cas protein/Molecular diagnosis引用本文复制引用
基金项目
深圳市科技计划项目(JCYJ20180307102005105)
出版年
2024
NETL
NSTL
万方数据