Research progress on molecular diagnostic applications based on CRISPR-Cas system
The CRISPR/Cas system is an adaptive immune system used by bacteria to defend against phage invasion.It is divided into two categories according to its effector proteins.In addition to specifically recognizing and cutting target sequences,it also has the property of trans-cutting surrounding single-stranded DNA and RNA molecules.This specific recognition mechanism has brought new opportunities for molecular diagnosis.This article briefly introduces the mechanism and types of CRISPR-Cas,focusing on the CRISPR-Cas system that has been used for molecular diagnosis,and lists its applications and related research progress.
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