Relationship between Blimp-1 expression in peripheral blood and Treg/Th17 imbalance and prognosis of aplastic anemia patients
Objective To investigate the relationship between the expression of B-lymphocyte in-duced maturation protein-1(Blimp-1)in peripheral blood and the imbalance of T regulatory cells(Treg)/T helper cell 17(Th17)and prognosis of aplastic anemia patients.Methods 140 patients diagnosed with aplastic anemia at the Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine from March 2020 to March 2023 were selected as the observation group,and 110 healthy volunteers who underwent physical exami-nations during the same period were selected as the control group.The expression level of Blimp-1 in peripheral blood,the quantities of Treg and Th17 cells,and the levels of interleukin-6(IL-6),interleukin-10(IL-10)and interleukin-17(IL-17)in serum were measured.The disease condition of the observation group was evaluated and divided into severe and mild cases.T tests were used to compare the difference of Blimp-1,Treg,Th17,in peripheral blood and IL-6,IL-10,IL-17 in serum between the two groups.Pearson test was used to analyze the correlation between Blimp-1 in peripheral blood and Treg,Th17,IL-6,IL-10,IL-17.The prognosis of aplastic anemia patients was followed up and the incidence of poor prognosis was calculated.K-M curve was used to compare the incidence of adverse prognoses between the two groups.Results The expression level of Blimp-1 in peripheral blood,Treg number,Treg/Th17 ratio and serum IL-10 level in the observation group were lower than those in the control group,and the Th17 number,serum IL-6 and IL-17 levels were higher in the observa-tion group compared to the control group,the difference was statistically significant(P<0.05).The expression level of Blimp-1 in peripheral blood of patients in the observation group was positively correlated with Treg num-ber,Treg/Th17 ratio and IL-10 level(r=0.351,0.376,0.328,P<0.05),and negatively correlated with Th17 number and IL-6 and IL-17 levels(r=-0.338,-0.409,P<0.05).The expression level of Blimp-1 in peripheral blood,Treg number,Treg/Th17 ratio and serum IL-10 level in severe patients were lower than those in mild pa-tients,and the Th17 number,serum IL-6 and IL-17 levels were higher than those in mild patients,the differ-ence was statistically significant(P<0.05).In the observation group,the incidence of poor prognosis in patients with high Blimp-1 expression was lower than that in patients with low Blimp-1 expression,the difference was statistically significant(P<0.05).Conclusion The decreased expression of Blimp-1 in the peripheral blood of patients with aplastic anemia may lead to an imbalance between Treg/Th17 cells,worsening the disease and re-sulting in a poor prognosis.
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