Relationship between PCBP1 and GPSM2 expression and EMT,clinicopathological fea-tures and prognosis in lung squamous cell carcinoma
Objective To investigate the relationship between the expression of poly (rC)-binding protein 1 (PCBP1) and G protein signal regulator 2 (GPSM2) in lung squamous cell carcinoma and epithelial-mesenchymal transition (EMT),clinicopathological features and prognosis.Methods Biopsy or surgical specimens were collected from 92 patients with lung squamous cell carcinoma who were admitted to the 984th Hospital of Joint Logistics Support Force,Rocket Force Characteristic Medical Center and Chizhou People's Hospital from January 2019 to January 2023.Reverse transcription polymerase chain reaction (RT-PCR) was used to detect and compare the expression of PCBP1 and GPSM2 mRNA in lung squamous cell carcinoma tis-sues and adjacent tissues.Additionally,the expression of PCBP1 and GPSM2 in lung squamous cell carcinoma tissues was analyzed in relation to EMT-related protein (β-catenin) and clinicopathological features.After 1 year of follow-up,the patients were divided into a good prognosis group and a poor prognosis group.The ex-pression of PCBP1 and GPSM2 mRNA in lung squamous cell carcinoma tissues was compared between the two groups.An ROC curve was drawn to evaluate the predictive value of PCBP1 and GPSM2 expression for poor prognosis in patients with lung squamous cell carcinoma.Results The expression of PCBP1 in lung squamous cell carcinoma was significantly lower than that in adjacent tissues,and the difference was statistically significant (t=6.502,P<0.05).The expression of GPSM2 was significantly higher than that in adjacent tissues,and the dif-ference was statistically significant (t=8.401,P<0.05).The positive expression of β-catenin membrane was found in lung squamous cell carcinoma and adjacent tissues,while the nuclear positive expression was only found in cancer tissues,and it was focal positive expression(P<0.05).Spearman correlation analysis showed that PCBP1 was negatively correlated with β-catenin expression in lung squamous cell carcinoma,and GPSM2 was positively correlated with β-catenin expression (r=-0.3286,0.445,P<0.05).The low expression of PCBP1 and the high expression of GPSM2 in lung squamous cell carcinoma were related to the degree of differentiation,N stage,M stage and lung metastasis (P<0.05).The level of PCBP1 mRNA in the poor prognosis group was lower than that in the good prognosis group,while the level of GPSM2 mRNA was higher than that in the good progno-sis group,and the difference was statistically significant (t=6.639,4.768,P<0.05).The ROC curve showed that the area under the curve (AUC) of PCBP1 combined with GPSM2 in predicting poor prognosis of patients with lung squamous cell carcinoma was the largest,which was 0.924.Conclusion The low expression of PCBP1 and the high expression of GPSM2 in lung squamous cell carcinoma are related to EMT-related proteins,tumor differentiation,N stage,M stage,pulmonary metastasis and prognosis.
万方数据
维普
