Analysis of T cell subsets and TLR4,TLR2,TP,ALB in SP patients infected with MRSA
Objective To investigate the changes of peripheral blood T-lymphocyte subpopula-tions in patients with methicillin-resistant staphylococcus aureus (MRSA) infected severe pneumonia (SP) and to analyze the diagnostic value of the combined peripheral blood toll-like receptor 4 (TLR4),toll-like receptor 2 (TLR2),and serum total protein (TP),albumin (ALB) assay for their diagnosis.Methods From January 2020 to December 2022,179 cases of MRSA infected SP patients were selected from Zhangye People's Hospi-tal affiliated with Hexi College as the infected group,and another 181 cases of MRSA uninfected SP patients were selected as the control group.The drug resistance of MRSA was analyzed,the levels of peripheral blood T lymphocyte subpopulations,TLR4,TLR2,and serum TP and ALB between the two groups were compared,and the value of peripheral blood TLR4,TLR2,serum TP,and ALB for diagnosis of MRSA infection with SP was analyzed.Results Antimicrobial drugs with high MRSA resistance rates include penicillin,benzoxiline,erythromycin,clindamycin,tetracycline,and ciprofloxacin.Antimicrobial drugs with complete sensitivity in-clude tigecycline,furotoxin,linezolid,and quinupristin.In comparison with the control group,peripheral blood CD19+,TLR4,and TLR2 levels were higher in the infected group (t=15.378,13.408,and 12.113,P<0.05),while levels of CD4+,CD3+,CD4+/CD8+,and serum TP and ALB were lower (t=23.295,26.320,and 17.810,P<0.05).The ROC curve was plotted and analyzed with the infected group as positive and the control group as negative.The results showed that the area under the curve (AUC) value of serum TLR4,TLR2,TP,and ALB combined test for diagnosis of MRSA infected SP was 0.932,which was higher than that of the four single tests (0.811,0.861,0.865,and 0.849,P<0.05).Conclusion Patients with MRSA-in-fected SP had abnormal changes in peripheral blood T-lymphocyte subpopulations and high expression of peripheral blood TLR4 and TLR2.Additionally,they showed low expression of serum TP and ALB.The com-bined detection of the four markers had a higher diagnostic value.
万方数据
维普
