Relationship between serum SAA,HMGB1,TNF-α expression and disease activity in patients with ankylosing spondylitis
Objective To explore the correlation between the expression of serum amyloid A (SAA),high migration group protein B1(HMGB1),tumor cell necrosis factor-α (TNF-α) and disease activity in patients with ankylosing spondylitis (AS),and analyze the value of these indicators in the diagnosis of AS and the evaluation of disease activity.Methods 152 AS patients admitted to the Rheumatology and Immunology Department of Xinyang Central Hospital from January 2021 to October 2023 were selected as the study group.According to bath ankylosing spondylitis disease activity index (BASDAI) score,the study group was divided into a disease active group and a disease remission group.And 80 patients with rheumatoid arthritis were selected as the control group.Serum C-reactive protein (CRP),SAA,HMGB1,TNF-α,erythrocyte sedimentation rate (ESR) and uric acid (UA) were compared between the two groups.The correlation between serum levels of SAA,HMGB1,TNF-α,and disease activity was analyzed using the Spearman method.The value of single and combined detection of serum SAA,HMGB1,and TNF-α in the diagnosis of AS was analyzed using the receiver operating curve (ROC).Results The levels of serum UA,CRP,ESR,SAA,HMGB1,and TNF-α in the study group were significantly higher than those in the control group,with statistical significance (t=5.827,50.112,50.329,35.944,46.683,67.298,P<0.05).Serum levels of SAA,HMGB1,and TNF-α in patients with the active stage were higher than those in patients with the remission stage,and the difference was statistically significant (t=10.977,13.302,12.705,P<0.05).Spearman analysis showed that the expression of SAA,HMGB1 and TNF-α in serum of AS patients were positively correlated with disease activity (r=0.743,0.684,0.571,P<0.005).The ROC curve demonstrated that when serum SAA,HMGB1,and TNF-α were combined,the AUC for predicting AS was 0.922,with a sensitivity of 92.76％ and specificity of 90.00％,better than single detection (P<0.05).Conclusion The levels of SAA,HMGB1,and TNF-α in the serum of patients with AS activity were significantly increased.SAA,HMGB1,and TNF-α showed a significant correlation with the disease activity of AS.The combined detection of the three markers could serve as a crucial reference for diagnosing AS and the evaluating disease activity.
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