Correlation between AMPK/mTOR activation and epithelial cell injury in chronic nasosinusitis
Objective The relationship between inflammation and AMPK/mTOR signaling pathway in chronic nasosinusitis was explored by constructing the epithelial cell injury model of chronic nasosinusitis.Methods Nasal mucosal epithelial cells were stimulated with different concentrations of TNF-α to induce rhinitis cell model The content of inflammatory factors in normal nasopharyngeal epithelial cells (normal group) and rhinitis cells (model group) was detected by Elisa,the apoptosis of nasopharyngeal epithelial cells in normal group and model group was detected by TUNEL,and the AMPK/mTOR signaling pathway related proteins (AMPK,p-AMPK,mTOR,MTOR) in normal group and model group were detected by WB.The relative expression of p-mTOR.Results Compared with 0 ng/mL and 1 ng/mL TNF-α treated rhinitis epithelial cells,P-AMPK protein was up-regulated in 10 ng/mL TNF-α treated cells,the difference was statistically significant (P<0.05),but the changes of AMPK protein at different concentrations were not statistically significant (P>0.05).Compared with the normal group,the contents of TNF-α and IL-1β in the model group were increased with statistical significance (P<0.05),the apoptosis rate was decreased with statistical significance (P<0.05),and the expression level of p-AMPK/AMPK protein was increased with statistical significance (P<0.05).P-mtor/mTOR protein expression levels were significantly decreased,and the difference was statistically significant (P<0.05).Conclusion TNF-α induces nasopharyngeal epithelial cell injury by activating AMPK/mTOR pathway.
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