The levels and clinical significance of serum lncRNA PVT1 and HIF-1α in patients with endometriosis
Objective To investigate the expression and clinical significance of LncRNA plasmacytoma variable translocation gene 1 (lncRNA PVT1) and hypoxia inducible factor-1α (HIF-1α) in endometriosis (Ems).Methods A total of 92 Ems patients treated at Chengdu Jinxinainan Hospital from October 2021 to July 2023 were selected as the study subjects (Ems group),meantime,92 patients who underwent hysterectomy for hysteromyoma in our hospital were regarded as the control group.The comparison of serum levels of lncRNA PVT1 and HIF-1α levels in patients from both groups and in different R-AFS staging of Ems was conducted.The relationships between lncRNA PVT1 and HIF-1α,and between R-AFS staging and lncRNA PVT1 and HIF-1α levels were analyzed.Receiver operating characteristic curve (ROC) analysis was applied to assess the value of lncRNA PVT1 and HIF-1α in detecting Ems.Results Compared to the control group,the expression levels of serum lncRNA PVT1 and HIF-1α in Ems patients were obviously higher,with statistical significance (P<0.05).As the progression of R-AFS stage,the expression levels of serum lncRNA PVT1 and HIF-1α in Ems patients gradually increased (P<0.05).Pearson correlation analysis showed that lncRNA PVT1 was positively correlated with HIF-1α (r=0.419,P<0.05);Spearman correlation analysis showed that the levels of serum lncRNA PVT1 and HIF-1α were obviously positively correlated with the R-AFS stage of Ems patients (r=0.467,0.699,P<0.05).The ROC curve showed that the AUC of lncRNA PVT1 and HIF-1α in diagnosing Ems alone was 0.915 and 0.873,the sensitivity was 87.0％ and 73.9％,and the specificity was 70.7％ and 73.9％,respectively,the AUC of the combined diagnosis of Ems was 0.962,the sensitivity was 87.0％,and the specificity was 78.3％;The proportion of patients with high expression of lncRNA PVT1 and HIF-1α in clinical stage Ⅲ to Ⅳ,deep growth,cyst size≥3 cm and dysmenorrhea was significantly higher than those with low expression of lncRNA PVT1 and HIF-1α,and the difference was statistically significant (P<0.05).Conclusion The expression levels of serum lncRNA PVT1 and HIF-1α in patients with Ems are increased,and the high expression of lncRNA PVT1 and HIF-1α is related to the R-AFS stage.Therefore,the combined detection of the two may be beneficial for the early targeted treatment in clinical settings.
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