Evaluation of screening methods for group B streptococcus in late pregnancy
Objective To evaluate the diagnostic performance of microbial culture,colloidal gold immunochromatography,real⁃time PCR and mass spectrometry in screening group B streptococcus(GBS).Methods A total of 1,506 perinatal pregnant women were admitted to the People's Hospital of Inner Mongolia Autonomous Region from January 2020 to December 2022.They were simultaneously screened using microbial culture,colloidal gold immunochromatography,fluorescent PCR and mass spectrometry rapid identification GBS.The enhanced culture method was used as the reference standard to compare the above methods.Results The detection rates were as follows:increased culture method,8.17％,direct culture method,3.12％,colloidal gold immunochromatography,5.17％,real⁃time fluorescence quantitative PCR method,11.49％,and mass spectrometry rapid identification method,7.17％.There were statistically significant differences between the detection rates of the different methods(P<0.05).The real⁃time fluorescence PCR method had the highest sensitivity(95.93％)and the lowest specificity(99.42％).The accuracy(98.61％)and specificity(99.78％)of the mass spectrometry rapid identification method were the highest,with a strong consistency Kappa value of 0.902.The sensitivity of colloidal gold immunochromatography was 63.41％,the specificity was 99.42％,and the accuracy was 96.48％.The sensitivity(32.52％)and accuracy(94.02％)of the direct culture method were the lowest.The minimum detection limit of real⁃time PCR and mass spectrometry was 1.5×103 CFU/mL.Conclusion Real⁃time fluorescent PCR and mass spectrometry are rapid identification methods with high sensitivity,accuracy and fast detection speed.They can be used for the rapid screening of GBS.Colloidal gold immunochromatography is suitable for large⁃scale preliminary screening in outpatient clinics and routine screening in primary hospitals.Although time⁃consuming,the bacterial culture method has high accuracy and a low rate of missed diagnosis and misdiagnosis.It is recommended that hospitals with the necessary conditions use it as a routine screening project.
Group B StreptococcusMicrobial culture methodColloidal gold immunochromatog-raphyReal-time PCREnhanced culture methodMass spectrometry rapid identification
万方数据
维普
