Expression and significance of lncRNA XIST and lncRNA TUG1 in peripheral blood of pa?tients with diarrhea predominant irritable bowel syndrome with damp heat constitution and non?damp heat constitution
Objective To compare the differences of lncRNA X inactive specific transcription fac⁃tor(lncRNA XIST)and lncRNA taurine upregulation gene 1(lncRNA TUG1)expression in peripheral blood be⁃tween dampness⁃heat and non⁃dampness⁃heat diarrheal irritable bowel syndrome patient and analyze its clinical significance.Methods 120 cases of diarrheal irritable bowel syndrome treated at Rugao Hospital of Traditional Chinese Medicine in Jiangsu Province from January 2021 to December 2023 were selected as the observation group.They were divided into two subgroups:dampness⁃heat subgroup and non⁃dampness⁃heat subgroup based on TCM constitution.100 healthy subjects who underwent physical examination during the same period were se⁃lected as the control group.The expression levels of lncRNA XIST and lncRNA TUG1 in peripheral blood and the level of C⁃reactive protein(CRP)in serum were detected and compared.The influencing factors and diag⁃nostic indicators of dampness⁃heat diarrheal irritable bowel syndrome were analyzed.Results The expression level of lncRNA XIST in the peripheral blood of the observation group was higher than that of the control group,and the expression level of lncRNA TUG1 was lower than that of the control group,with statistical significance(t=8.136,9.324,P<0.05).Within the observation group,the expression level of lncRNA XIST in peripheral blood and the CRP level in serum of the dampness⁃heat subgroup were higher than those in the non⁃dampness⁃heat subgroup,and the expression level of lncRNA TUG1 was lower than that in the non⁃dampness⁃heat sub⁃group,with statistical significance(P<0.05).LncRNA XIST was identified as a risk factor for dampness⁃heat diar⁃rheal irritable bowel syndrome,and lncRNA TUG1 was found to be a protective factor for dampness⁃heat diar⁃rheal irritable bowel syndrome(P<0.05).The areas under the ROC curves of lncRNA XIST and lncRNA TUG1 in peripheral blood for the diagnosis of dampness⁃heat diarrheal irritable bowel syndrome were 0.754(P<0.05)and 0.810(P<0.05),respectively.Conclusion The increased expression of lncRNA XIST and the decreased expres⁃sion of lncRNA TUG1 are associated with dampness⁃heat diarrheal irritable bowel syndrome.The levels of these two indicators in peripheral blood have diagnostic value for dampness⁃heat diarrheal irritable bowel syndrome.
万方数据
维普
