The role of IL?10 and HMGB1 levels in alveolar lavage fluid combined with mNGS sequencing in the prognostic assessment of severe pneumonia
Objective To investigate the levels of interleukin⁃10(IL⁃10)and high mobility group protein B1(HMGB1)in alveolar lavage fluid in conjunction with metagenomic next⁃generation sequencing(mNGS).sequencing in the prognostic assessment of severe pneumonia.Methods Ninety⁃seven patients with severe pneumonia admitted to our hospital from July 2020 to December 2023 were selected for the study.The aim was to analyze the prognosis of patients with severe pneumonia by examining the general data of those in the good prognosis group and the poor prognosis group,as well as identifying the risk factors that affect prognosis.Additionally,the study compared the levels of IL⁃10,HMGB1,and the positivity rate of mNGS between the two groups.The study also aimed to calculate the predictive value of IL⁃10,HMGB,and mNGS individually and in combination for predicting the prognosis of patients with severe pneumonia.Results After 28 days of treatment,there were 39 cases in the poor prognosis group and 58 cases in the good prognosis group.A compari⁃son of CIPS grading between the two groups showed statistically significant differences(P<0.05).The IL⁃10,HMGB1 levels and mNGS positivity rate were significantly higher in the poor prognosis group than in the good prognosis group(P<0.05).As shown by binary logistic regression analysis,CIPS rated as heavy,the levels of L⁃10 and HMGB1 increased,and the positive rate of mNGS increased were risk factors affecting the prognosis of severe pneumonia(P<0.05).The AUC of IL⁃10,HMGB1,and mNGS combined to predict the prognosis of patients with severe pneumonia was 0.894,which was higher than that of IL⁃10,HMGB1,and mNGS alone(P<0.05).Conclusion IL ⁃ 10 in alveolar lavage fluid,HMGB1 levels joint mNGS sequencing play a signifi⁃cant role in evaluating the prognosis of severe pneumonia.They can predict the prognosis of patients with clini⁃cal conditions and provide strong support.
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