Serum levels of DAO and TLR9 expression in patients with advanced colon cancer and their relationship with intestinal flora disturbance and chemotherapy efficacy
Objective To investigate serum levels of diamine oxidase(DAO)and Toll-like recep-tor 9(TLR9)expression in patients with advanced colon cancer and their relationship with intestinal flora dis-order and chemotherapy efficacy.Methods A total of 120 cases with advanced colon cancer who received chemotherapy from January 2023 to December 2023 were selected as the study group.Additionally,123 healthy individuals who underwent physical examinations during the same period were chosen as the control group.Serum levels of DAO and TLR9 were measured and compared between the two groups.Fecal samples from the study group were collected to analyze their intestinal flora composition.Based on the assessment crite-ria for intestinal flora imbalance,the groups were categorized accordingly.The correlation between intestinal flora imbalance and colon cancer was examined using the Pearson method.The effectiveness of chemotherapy was evaluated through the receiver operating characteristic curve(ROC).Results The levels of DAO and TLR9 in the study group were higher than those in the control group,and the differences were statistically sig-nificant(P<0.05).The expression levels of DAO and TLR9 in serum were as follows:flora dysregulation Ⅱgroup>flora dysregulationⅠgroup>intestinal normal group,the difference was statistically significant(P<0.05).The number of Escherichia coli and fecal coccus:flora disorder Ⅱ group>flora disorder Ⅰ group>intestinal normal group;the number of lactobacillus and bifidobacterium:flora disorder Ⅱ group<flora dis-order Ⅰ group<intestinal normal group,the difference was statistically significant(P<0.05).Serum DAO and TLR9 levels in advanced colon cancer patients were positively correlated with Escherichia coli and fecal coccus,and negatively correlated with Lactobacillus and bifidobacterium(P<0.05).The levels of DAO and TLR9 in patients with advanced colon cancer in the ineffective group were higher than those in the remission group,and the difference was statistically significant(P<0.05).Conclusion Serum DAO and TLR9 levels were increased in advanced colon cancer patients,which were closely related to intestinal flora imbalance and chemotherapy efficacy.However,these levels did not prove to be a reliable diagnostic indicator for advanced colon cancer patients undergoing chemotherapy.
NETL
NSTL
万方数据
