Determination of 7 Synthetic Cannabinoids in Blood and Urine by High Performance Liquid Chromatography-Tandem Mass Spectrometry
An analytical method for the simultaneous determination of synthetic cannabinoids(SCs)in blood and urine was established using high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Acetonitrile containing 0.1％formic acid was used as the precipitation protein and ex-traction solvent.7 kinds of SCs in blood and urine were determined by HPLC-MS/MS after protein precipi-tation,ultrasonic extraction and centrifugation.Using 0.1％(v/v)formic acid aqueous solution(contai-ning 5 mmol/L ammonium formate)and acetonitrile as the mobile phase,gradient elution was performed.Qualitative and quantitative analysis were conducted by positive ion ionization mode of electron spray ioni-zation(ESI+)and multiple reaction monitoring mode.The results showed that 7 kinds of SCs had good linearity within the calibration range of 0.1～20 ng/mL(r＞0.998).The average recoveries of the 7 SCs in blood and urine at 3 spiked levels were in the range of 66.3％～104.8％and 69.1％～96.8％,respec-tively.The limits of detection were in the range of 0.030～0.117 ng/mL and 0.011～0.051 ng/mL,re-spectively.The limits of quantification were in the range of 0.100～0.390 ng/mL and 0.037～0.169 ng/mL,respectively.The relative standard deviations of intra-day precision were 1.2％～11.4％and 1.0％～10.9％,respectively.The relative standard deviations of inter-day precision were 1.6％～10.3％and 1.3％～11.2％,respectively.The matrix effects ranged from 76.1％to 97.7％and 75.2％to 93.3％,re-spectively.The established method was fast and simple.Mice experiments have shown that synthetic can-nabinoid precursors were detected within 1 hour after administration.Therefore,the method is suitable for screening and confirming abusers of synthetic cannabinoids.
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