首页|二倍体紫苏线粒体基因组特征及其系统发育分析

二倍体紫苏线粒体基因组特征及其系统发育分析

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
[目的]通过注释和组装二倍体紫苏线粒体基因组，旨在丰富其组学数据，为紫苏的种质鉴评和品种选育提供参考。[方法]利用Illumina和PacBio数据的组合，对二倍体紫苏的整个线粒体基因组进行了测序、注释和组装。[结果]组装得到的二倍体紫苏线粒体基因组长301 156 bp，具有典型的环状结构。基因组的GC含量为45。23％，共鉴定出59个独特基因，包括37个编码蛋白质的基因、20个tRNA基因和2个rRNA基因，其中8个编码蛋白质的基因含有18个内含子。二倍体紫苏线粒体基因组的密码子显示出明显的A/T偏好。检测到180个分散重复序列、78个简单序列重复和6个串联重复序列。此外，70个序列片段(12 680 bp)已从叶绿体转移到线粒体基因组，占整个线粒体基因组的4。23％。系统发育分析显示，在唇形科植物中，二倍体紫苏与丹参和Platostoma chinense最为密切相关。种间Ka/Ks比较表明，28个编码蛋白质的基因的Ka/Ks＜1。[结论]本研究充实了二倍体紫苏线粒体基因组数据，可为其种质资源鉴评、新品种选育等研究提供理论依据。
Mitochondrial genomic characterization and phylogenetic analysis of Perilla citriodora(Lamiaceae)
[Purpose]This study aimed to enrich the genomic data of Perilla citriodora by annotating and assembling its mitochon-drial genome,thereby providing a reference for germplasm evaluation and the breeding of new varieties of this diploid species.[Method]The entire mitochondrial genome of Perilla citriodora was sequenced,annotated,and assembled using a combination of Illumina and PacBio datasets.[Result]The assembled mitochondrial genome was 301 156 bp long,with a GC content of 45.23％,and exhibited a typical circular structure.In total,59 unique genes were identified,including 37 protein-coding genes,20 tRNA genes,and 2 rRNA genes.Among the protein-coding genes,eight contained 18 introns.The mitochondrial genome indicated a high preference for A/T codon usage.Furthermore,180 dispersed repeat sequences,78 simple sequence repeats,and 6 tandem repeat sequences were detected.Additionally,70 sequence fragments(12 680 bp)were transferred from the chloroplast to the mitochondri-a,accounting for 4.23％of the entire mitochondrial genome.Phylogenetic analysis revealed that within the Lamiaceae family,Perilla citriodora was most closely related to Salvia miltiorrhiza and Platostoma chinense.Interspecies comparisons indicated that the Ka/Ks ratio of the 28 protein-coding genes was less than 1.[Conclusion]The results of this study enhance the mitochondrial genome data of Perilla citriodora and provide a theoretical basis for germplasm resource evaluation and the breeding of new varieties of this diploid species.

Perilla citriodorasequence characteristicsphylogenetic analysisrelative synonymous codon usageRNA editingmitochondrion

王茹、罗永坚、邱道寿

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广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室,广东广州 510640

湖北民族学院林业与园艺学院,湖北恩施 445000

二倍体紫苏 序列特征 系统发育分析 RSCU RNA编辑 线粒体

广东省省级科技计划项目广东省科技计划项目广东省林业科技创新项目

2019B0303160082023B12120600382021KJCX013

2024

10.13321/j.cnki.subtrop.agric.res.2024.02.002

亚热带农业研究
福建农林大学

亚热带农业研究

CSTPCD
影响因子：0.517
ISSN：1673-0925
年,卷(期)：2024.20(2)