[Purpose]The objective of this study was to investigate the composition of SPL gene family members in Euscaphis kon-ishii Hay ata and their expression pattern of SPL gene in flowers and fruits of E.konishii.[Method]A bioinformatic approach was used to systematically identify and analyse the EjSPLs.[Result](1)The results showed that a total of 26 EjSPLs genes with SBP-conserved structural domains were identified in the E.konishii genome,which were non-uniformly distributed on 12 chromosomes,and 13 pairs of genes were found to undergo segmental duplication.(2)Cis-element analysis showed that EjSPLs genes were regula-ted by light and phytohormones.(3)The EjSPLs were classified into eight subfamilies by phylogenetic analysis,and members of the same subfamily had similar gene structures and conserved motifs.(4)Expression pattern analysis showed that EjSPLs genes were tis-sue-specific,but most members of the same subfamily had similar expression patterns;moreover,20 EjSPLs genes were mainly ex-pressed in the floral organs or fruits,and seven of them were only expressed at the green,colour change and red fruiting stages.[Conclusion]The results of this study provide comprehensive information on the SPL gene family of E.konishii,reveal the expres-sion pattern of EjSPL in flowers and fruits,which lay a theoretical foundation for elucidating the biological process of SPL genes.