Objective To explore the histological evidence of Didang Decoction mediating hepatocellular carcinoma invasion,metastasis and portal vein tumor thrombosis.Methods According to the literature reports and previous studies,two highly invasive hepatocellular carcinoma cell lines,HepG2 and HCCLM3,human hepatocellular carcinoma cells were selected respectively;the cell con-centration was adjusted,and the nude mice were injected with 3 x 106 cancer cells(both 300μL)in the lower peritoneal cavity,in order to establish the hepatocellular carcinoma portal vein tumor thrombus model.The model was identified and replicated,and randomly di-vided into 4 groups,namely,HepG2 and HCCLM3 saline group(control group),HepG2 and HCCLM3 Didang Decoction group,6 mice in each group,and started to be administered by gastric gavage in the 2nd week after intraperitoneal injection of cancer cells,and the control group was given the same amount of saline,for 5 consecutive weeks.The four groups of nude mice were compared in terms of general status,tumorigenicity of the omentum and liver,vascular invasion and portal vein microtumor thrombosis,and immunohistochem-istry was used to observe the expression of vascular endothelial growth factor(VEGF)and epidermal growth factor receptor(EGFR).Results The general state of nude rats did not change significantly at the early stage of intraperitoneal inoculation,and by the 5 th week of drug administration,the rates of retinal tumorigenesis and liver tumorigenesis of the two types of hepatocellular carcinoma cells in the saline group were comparable,and the proportion of retinal vascular invasion of HCCLM3 cells was higher than that of HepG2 cells,P＜0.05,and the incidence of portal microtumor thrombosis of HCCLM3 cells was significantly higher than that of HepG2 cells,P＜0.05.The rate of retinal tumorigenesis,liver tumorigenesis and retinal vascular invasion of HCCLM3 cells of the Didang Decoction group were similar to those of the saline group.The rate of retinal tumorigenesis,hepatic tumorigenesis,vascular invasion,microtumor thrombus and VEGF,EGFR expression in HCCLM3 cells in the Didang Decoction group was significantly lower than that in the saline group,P＜0.05,and the rate of retinal morigenesis in HepG2 cells in the Didang Decoction group was also reduced.Conclusion Didang Decoc-tion can significantly inhibit liver metastasis and portal vein thrombus formation of epatocellular carcinoma cells,and inhibit tumor neovascularization by its acute effect of breaking blood and expelling blood stasis,which may make tumor micro-metastatic foci dormant for a long period of time.
维普
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