基于光诱导电子转移的比率型发光传感器定量检测生物样本中的多巴胺

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国家科技期刊平台
NETL
NSTL
万方数据
维普

中文摘要：长余辉发光材料是一种光致发光材料,在激发停止后以光的形式将储存的能量缓慢释放出来,因其优异的光学性能而在生物传感和生物成像领域得到广泛应用.本文将发光性能优异的双发射长余辉纳米粒子Sr2.992Mg0.9Si2O8∶0.008Eu2+,0.10Mn2+(SEM)和酪氨酸酶(Tyrosinase,TYR)共价偶联,开发了一种比率型发光传感器(SEM-TYR),其可避免自发荧光干扰和外部干扰,可用于高灵敏、高选择性检测生物样本中的多巴胺(DA).当DA存在时,酪氨酸酶将其氧化生成多巴醌,依据光诱导电子转移(PET)原理,SEM-TYR光致激发的电子部分转移至电子受体多巴醌,导致SEM-TYR中680 nm红色余辉猝灭.在最优实验条件下,双发射余辉猝灭比值Δ(I680/I460)与DA浓度呈良好的线性关系,线性范围为0.2～10 μmol/L(R2=0.9965),检出限为40 nmol/L.将该方法用于生物样本血清、尿液及汗液中DA的检测,回收率分别为94.8%～104.0%,91.3%～101.6%和97.4%～105.2%,RSD<6.8%(n=10).所开发的比率型发光传感器用于生物样本中DA的检测具有潜在的应用前景.

外文标题：A Ratiometric Luminescent Sensor Based on Photoinduced Electron Transfer for Quantitative Detection of Dopamine in Biological Samples

外文摘要：The persistent luminescence particle material is a type of photoluminescent material that gradually emits stored energy in the form of light once excitation ceases.Owing to its exceptional optical properties,it finds extensive applications in biosensing and bioimaging.In this work,a ratiometric luminescent sensor(SEM-TYR)was developed by covalent coupling of dual-emission long-afterglow nanoparticles Sr2.992Mg0.9Si2O8:0.008Eu2+,0.10Mn2+(SEM)with exceptional luminescence performance and tyrosinase(TYR).This innovative design effectively eliminates spontaneous fluorescence interference and external disturbances,enabling highly sensitive and selective detection of dopamine(DA)in biological samples.When DA is present,tyrosinase facilitates its oxidation to dopaquinone,and in accordance with the principle of photoinduced electron transfer(PET),the photoactivated electron moiety of SEM-TYR is transferred to the electron acceptor dopaquinone,resulting in the attenuation of the 680 nm red afterglow observed in SEM-TYR.Under optimized conditions,the ratio Δ(I680/I460)showed a good linear relationship with the dopamine concentration in the range of 0.2-10 μmol/L and the limit of detection limit was 40 nmol/L.The developed method was applied for the detection of dopamine in human serum,urine and sweat after being verified by standard dopamine.Good agreement was achieved and the quantitative recovery was in the range of 94.8％—104.0％,91.3％—101.6％and 97.4％—105.2％,respectively.The RSD was less than 6.8％(n=10).The ratiometric lumines-cent sensor developed in this study has a potential application prospect in the determination of DA in biological samples.

外文关键词：

Ratiometric luminescent sensorDopaminePersistent luminescence nanoparticlePhotoinduced electron transferAutofluorescence-free

作者：

朱润芝、王怡、纳佳雪、曹乐乐、张辉、王迎辉、孟哲

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作者单位：

宁夏大学化学化工学院,省部共建煤炭高效利用与绿色化工国家重点实验室,银川 750021

关键词：

比率型发光传感器多巴胺长余辉纳米粒子光诱导电子转移无自发荧光

基金：

国家自然科学基金国家自然科学基金宁夏回族自治区自然科学基金宁夏回族自治区自然科学基金宁夏大学生创新创业训练计划

项目编号：

21966024217680032023AAC030182022AAC03045S202310749146

出版年：

2024

DOI：

10.7503/cjcu20230391

高等学校化学学报

中华人民共和国教育部委托吉林大学和南开大学

高等学校化学学报

CSTPCD北大核心

影响因子：1.067

ISSN：0251-0790

年,卷(期)：2024.45(1)

参考文献量36