Metal-free Melanin Nanoenzymes for the Treatment of Liver Fibrosis
Metal-free Melanin nanoenzymes(MeNPs)were synthesized via the free radical polymerization of 5,6-dihydroxyindole as monomer,and its therapeutic effect on liver fibrosis was explored.The results showed that the MeNPs nanoenzymes had monodisperse spherical structure with a particle size of(91.3±2.6)nm,with superoxide-like dismutase(SOD)and catalase-like(CAT)activities,and their catalytic activities followed typical Michaelis-Menten kinetics with Michaelis-Menten constant(Km)and maximum reaction velocity(Vmax)of 1.01 mmol/L and 8.49×10-6 mol/(L·s),respectively.In vitro cell experiments such as cell counting kit-8(CCK-8)confirmed that MeNPs had cytoprotective effects on H2O2-induced oxidative stress.Inflammatory factors IL-6,TNF-α,IL-1β and chemokine CXCL-1 were significantly reduced in the mouse model of liver fibrosis treated by MeNPs nanoenzymes.Liver damage indexes ALT,AST and H&E staining of liver tissue showed the good efficacy of MeNPs in liver fibrosis.The research has guiding significance for the construction of safe and efficient free radical scavenging nanoenzymes,and also provides a theoretical basis and material basis for the treatment of liver fibrosis.
国家科技期刊平台
NSTL
万方数据
维普
