Preparation of Macroporous Weak Cation Exchange Chromatography Medium with High Capacity through"Grafting from"Method
Macroporous polymer chromatography medium has a low protein binding capacity due to its large pore size and low surface area.To solve this problem,the weak cation exchange chromatography medium with high capacity was prepared through the grafting polymerization of methacrylic acid on the macroporous polyacrylate microspheres,which was initiated by redox reaction.The reaction factors were evaluated and the general rules between these factors and proteins binding capacity were obtained,including monomer concentration,potassium persulfate concentration and reaction temperature.The static binding capacity and dynamic binding capacity of the obtained medium reached 252.21 and 157.25 mg/mL,respectively.It was also found that the medium with a certain ion exchange capacity could maintain 100 mg/mL protein binding capacity in 0.2 mol/L NaCl buffer.The chromatographic medium was used for the purification of lysozyme in egg white,and high purification efficiency was obtained.
Weak cation exchange chromatography mediumMacroporous polymerProtein binding capacityGrafting from methodSeparation and purification
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