Rapid Determination of D-(+)-Glucose in Urine Using Surface-enhanced Raman Spectroscopy
The silver(Ag)snowiness micro-nanostructures with a large aspect ratio was prepared by the polyol reduc-tion method,and it was used as the substrate for surface enhanced Raman scattering(SERS).Rhodamine 6G(R6G)was used as the probe molecule to study the SERS effect of Ag nanowires.The results showed that the minimum detection concentration of R6G on Ag nanowires was 10‒10 mol/L,and the enhancement factor(EF)was about 1.0×104.The relative standard deviation(RSD)calculated based on the scattering peak intensities of 610 and 772 cm‒1 was 14.66％and 13.75％,respectively,indicating that the SERS signal intensity distribution of R6G on Ag nanowires substrate was relatively uniform to lead to good signal reproducibility.In addition,Ag nanowires were used as SERS substrates to detect low levels of D-(+)-glucose in urine.It was found that the detection limit of Ag nanowires for D-(+)-glucose concentration was as low as 10‒6 mg/mL,and the RSD value was less than 8％,indicating that Ag nanowires had good sensitivity and signal reproducibility for trace detection of D-(+)-glucose.This study could provide some technical support for blood glucose monitoring and early prevention of diabetes.
万方数据
维普
