Preparation of NPCl-CDs/Fe3+Fluorescent Probe and the Detection for L-Cys
In this work,a blue-green NPCl-CDs was synthesized by one-step hydrothermal method using glucose,ethylenediamine,concentrated hydrochloric acid and concentrated phosphoric acid as reaction precursors.Then NPCl-CDs/Fe3+fluorescent probe was constructed for the quantitative detection of L-Cys in actual samples.The fluorescence intensity of NPCl-CDs could be quenched after addition of Fe3+.The fluorescence intensity of the NPCl-CDs/Fe3+system was restored when L-Cys was introduced into the system.Therefore,a novel"on-off-on"NPCl-CDs/Fe3+-L-Cys fluorescence sensing system for L-Cys quantitative detection was constructed.This experiment showed a wide linear region in the concentration range of 5.8-60.0 μmol/L with a detection limit of 0.052 μmol/L.The results indicated that the fluorescence sensing system had good selectivity,sensitivity and potential application value for the detection of L-Cys in real samples.
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