首页|Expression of Hepatitis B Surface Antigen Gene in Ginseng Cells
Expression of Hepatitis B Surface Antigen Gene in Ginseng Cells
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The recombinant plasmid pBIBSa containing the HBsAg DNA fragment was transferred into Agrobacte-rium tumefaciens strain LBA4404 directly. Ginseng cells were transfected with A. Tumefaciens carrying pBIBSa and the ginseng cell lines carrying HBsAg-S gene were obtained. The presence of target gene in the transfect cells was confirmed by PCR and RT-PCR. A clear band at the site of 700 bp was observed by agarose electrophoresis analysis of the samples containing the target gene. HBsAg expressed by the transgenic ginseng cells was detected by Western blot. Maximum expression levels of 184 ng HBsAg/g FW and 0. 009% of the total soluble proteins were observed by ELISA. HBsAg in ginseng cells was located both on the cell membrane and in the nuclei.
Transgenic cellHBsAgGinseng
YU Hai-peng、XUE Yan、AN Wei、LIU Dan、HAO Shu-mei、SHENG Jun
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College of Life Science,Jilin University,Changchun 130012,P.R China
Organ Transplantation Department of First Hospital,Jilin University,Changchun 130021,P.R.China
Changchun Institute of Biological Products,Changchun 130062,P.R.China
Changchun University of Science and Technology,Changchun 130022,P.R.China
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Science and Technology Development Planning Foundation of Jilin Province of China
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