Objective To study the effect and feasibility of accurate liver resection guided by model of liver three dimensional reconstruction (3D) and model of simulating hepatectomy with iCT images. Methods Forty-seven patients suffered from liver cancer and undergone liver resection,hospitalized from Jun. 2008 to Dec. 2009 were divided into iCT group (n=23) and routine group (n=24). Pre-operation,the volume and mor-phous of total liver were reconstructed with 3D using images of iCT in iCT group patients,and simulating hepatectomy models were built according the principle of liver segments dissection. Liver resection practice was guided by the 3D images and simulating hepatectomy models. Patients in routine group underwent liver resection with two-dimension images. Results Compared with routine group,the volume of intraoperative blood loss,blood transfusion,and operative duration,in iCT group were remarkably less than that in routine group (P<0.05),the rate of blood transfusion was not different (P>0.05) between two groups. Recovery of ALT post-operation in iCT group was more better than that in routine group (P<0.05),while the recovery of alpha-fetoprotein (AFP) and total bilirubin (TB) were not different remarkably between the two groups (P>0.05). Patients were followed up from 3-18 (12.6±2.3)months. Survival rate of iCT group (100%) was significant higher than that in routine group (78.3%)(P=0.000);while carcinoma recurrence rate in iCT group (18.1%) was more less than that in routine group(47.8%)(P=0.035). Conclusion Liver resection for the treatment of hepatocellular carcinoma (HCC),guided by 3D reconstruction and simulating hepatectomy model,will promote the clinic treating effect of HCC.