Study on the effect of berberine hydrochloride combined with cefoperazone-sulbactam on biofilm formation of carbapenem-resistant Pseudomonas aeruginosa
Objective To investigate the inhibitory effect of berberine hydrochloride(BBH)combined with cef-operazone-sulbactam(CSL)on biofilm formation of carbapenem-resistant Pseudomonas aeruginosa(CRPA).Methods Ten CRPA strains isolated from clinical samples were selected.The minimum inhibitory concentration(MIC)of BBH and CSL,both alone and in combination,was determined using the microdilution method and checkerboard microdilution method.The fractional inhibitory concentration index(FICI)was calculated.Crystal violet staining was used to screen biofilm-producing strains.Strain PA1 10 was chosen for further experiments to assess the inhibitory and biofilm-elimina-tion effects of BBH combined with CSL.Results The MIC range for BBH was 512-2 048 mg/L,while for CSL it was 4-128 mg/L.The FICI for BBH combined with CSL ranged from 0.268 to 0.625,indicating synergy in 4 strains and ad-ditive effects in 6 strains.All 10 strains produced biofilms,with varying intensities.In biofilm inhibition experiments,the inhibition rate was 50.7％for BBH(512 mg/L),62.8％for CSL(1 mg/L),and 88.2％for the combination,signifi-cantly higher than the single agents(P＜0.01).In biofilm elimination experiments,the inhibition rates were 44.5％(BBH,512 mg/L),56.7％(CSL,1 mg/L),and 80.9％(combination),again showing significant improvement with the combination(P＜0.01).Conclusion BBH combined with CSL effectively enhances the antibacterial activity against CRPA and inhibits and eliminates biofilm formation,providing new possibilities and evidence for clinical treatment of CRPA.
berberine hydrochloridecefoperazone-sulbactamcarbapenem resistant Pseudomonas aeruginosabiological film
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