Expression of SIRT1 and NF-κB in degenerated disc tissue in lumbar disc herniation and its correlation with de-generation severity
Objective To investigate the expression of silent information regulator 1(SIRT1)and nuclear factor-kappa B(NF-κB)in the nucleus pulposus tissue of degenerated discs in patients with lumbar disc herniation(LDH)and analyze their correlation with the degree of degeneration.Methods A total of 124 LDH patients undergoing surgical treatment from May 2022 to February 2024 were prospectively selected.The patients were categorized into mild,moder-ate,and severe degeneration groups according to the Pfirrmann grading system.The levels of SIRT1 and NF-κB expres-sion in the nucleus pulposus tissue were measured and compared across groups.Pearson's correlation analysis was used to examine the relationship between SIRT1,NF-κB expression,and Pfirrmann grades.Ordinal regression analysis was ap-plied to determine the impact of SIRT1 and NF-κB on the degree of disc degeneration.Results SIRT1 expression de-creased,while NF-κB expression increased with higher Pfirrmann grades(P＜0.05).Pearson's correlation analysis in-dicated a negative correlation between SIRT1 and Pfirrmann grades(r=-0.437,P＜0.001)and a positive correlation between NF-κB and Pfirrmann grades(r=0.493,P＜0.001).Ordinal regression analysis identified disc pathology(sequestration type),age,body mass index(BMI),NF-κB expression,and SIRT1 expression as independent predic-tors of disc degeneration severity(OR＞1,P＜0.05).The model fit was validated with a-2 log-likelihood x2=94.026(P＜0.001)and a goodness-of-fitx2=286.001(P=0.018),indicating good model adequacy.Conclusion In LDH patients,the degeneration of lumbar discs is characterized by reduced SIRT1 expression and increased NF-κB expression.Both markers are closely associated with the degree of lumbar disc degeneration,providing potential targets for therapeutic intervention.
lumbar disc herniationsilent information regulator 1nuclear factor-kappa Bdisc degeneration
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