Analysis of KIR gene polymorphism in Han blood donors from Hunan Province
Objective To investigate the genotype and polymorphism of killer immunoglobulin-like receptors(KIR)in Han blood donors from Hunan Province.Methods Peripheral blood samples were collected from 332 unrelat-ed Han blood donors randomly selected from the database of the Changsha Blood Center.Fluorescent PCR combined with Taqman probe technology was employed for qualitative genotyping of 16 known KIR genes.Gene frequencies,genotypes,and haplotypes were analyzed according to the Allele Frequency Net Database(AFND).Statistical analysis of KIR gene frequencies was performed using SPSS 19.0 software and x2 test.Results All 332 individuals expressed KIR2DL4,3DL2,and 3DL3 genes.The most prevalent genes included KIR2DL1(99.70％),2DP1(99.70％),3DP1(99.70％),2DL3(99.10％),2DS4(96.99％),and 3DL1(96.39％),while less common genes were KIR2DS3(19.28％),2DL2(21.39％),2DS2(21.69％),and 2DS5(21.69％).A total of 29 genotypes were identified,in-cluding 4 AA genotypes.The most frequent genotype was ID1.Fourteen genotypes were observed only once in this cohort.Conclusion The distribution of KIR genotypes in the Han population of Hunan Province is relatively concentrated,dis-playing similarities with other Han populations in China.However,distinct polymorphic characteristics were identified,demonstrating notable genetic diversity.
killer immunoglobulin-like receptorgenetic polymorphismgene frequency
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