湖南地区汉族无偿献血人群中KIR基因多态性研究
Analysis of KIR gene polymorphism in Han blood donors from Hunan Province
陈洁 1阳智芬 1曹丽群 1蒋义 1康甜 1谢毓滨1
作者信息
- 1. 长沙血液中心输血研究所(湖南长沙 410001)
- 折叠
摘要
目的 调查湖南地区汉族无偿献血人群中KIR基因型和基因多态性.方法 在长沙血液中心无偿献血者库中随机抽取湖南籍汉族无血缘关系的332例志愿者外周血,应用荧光PCR法结合Taqman探针技术对人类自然杀伤细胞免疫球蛋白受体(KIR)16种基因进行定性分型检测,参照等位基因频率网络数据库(AFND)的命名,检测基因型及单体型组成,采用统计软件SPSS 19.0对人群间KIR的16种基因频率进行x2x检验.结果 332例检出16种已知KIR基因,所有样本均表达KIR2DL4、3DL2、3DL3基因;其中分布较广的为 KIR2DL1(99.70％)、2DP1(99.70％)、3DP1(99.70％)、2DL3(99.10％)、2DS4(96.99％)、3DL1(96.39％);分布较低的为 KIR2DS3(19.28％)、2DL2(21.39％)、2DS2(21.69％)、2DS5(21.69％).共发现 29种基因型,其中AA基因型有4种,最常见的基因型为ID1.有14种基因型在本次检测的湖南汉族人群中出现一次.结论 湖南省汉族人群的KIR基因型分布相对集中,与其他地区汉族人群大致相似,但也具有其明显的特点,具有一定的多态性.
Abstract
Objective To investigate the genotype and polymorphism of killer immunoglobulin-like receptors(KIR)in Han blood donors from Hunan Province.Methods Peripheral blood samples were collected from 332 unrelat-ed Han blood donors randomly selected from the database of the Changsha Blood Center.Fluorescent PCR combined with Taqman probe technology was employed for qualitative genotyping of 16 known KIR genes.Gene frequencies,genotypes,and haplotypes were analyzed according to the Allele Frequency Net Database(AFND).Statistical analysis of KIR gene frequencies was performed using SPSS 19.0 software and x2 test.Results All 332 individuals expressed KIR2DL4,3DL2,and 3DL3 genes.The most prevalent genes included KIR2DL1(99.70％),2DP1(99.70％),3DP1(99.70％),2DL3(99.10％),2DS4(96.99％),and 3DL1(96.39％),while less common genes were KIR2DS3(19.28％),2DL2(21.39％),2DS2(21.69％),and 2DS5(21.69％).A total of 29 genotypes were identified,in-cluding 4 AA genotypes.The most frequent genotype was ID1.Fourteen genotypes were observed only once in this cohort.Conclusion The distribution of KIR genotypes in the Han population of Hunan Province is relatively concentrated,dis-playing similarities with other Han populations in China.However,distinct polymorphic characteristics were identified,demonstrating notable genetic diversity.
关键词
自然杀伤细胞免疫球蛋白受体/基因多态性/基因频率Key words
killer immunoglobulin-like receptor/genetic polymorphism/gene frequency引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
万方数据