Effects of exogenous ABA and Ca2+ on sucrose metabolism in grape (Vitisvinifera L.) seedlings under low temperature treatment and recovery
In order to figure out the responsing mechanisms of exogenous abscisic acid (ABA) and Ca2+ on sucrose metabolism and key enzymes under low temperature,we measured the contents of sucrose,glucose,fructose and starch,the activities of sucrose-phosphate synthase (SPS),sucrose synthase (SS),acid invertase (AI) and neutral invertase (NI) in Cabemets (Vitisvinifera L.) leaves at 5℃ and recovery at 25℃ treatment.The results showed with the increase in concentrations of ABA or Ca2 +,the contents of sucrose,glucose,fructose and starch were increased significantly,and they were at the maximums when the concentrations of ABA or Ca2+ were 5 mg·L-1 or 15 mmol·L-1,resulting in increases of 67.88％,21.8％,213.77％,7.04％,51.38％,2.35％,8.84％,and 31.81％,or 72.81％,35.37％,123.57％,36.44％,25.90％,28.67％,210.56％,and 210.90％,respectively;The activities of acid invertase (AI),neutral invertase (NI),sucrose-phosphate synthase (SPS) and sucrose synthase (SS) were also increased markedly by 64.10％,125.00％,193.29％,756.31％,2 291.51％,1 569.31％,1 523.13％,and 1 336.77％,or 26.42％,20.38％,30.75％,58.30％,111.63％,14.14％,14.32％,and 66.39％,respectively.Our results provided evidence that exogenous ABA or Ca2 + may impose damages to grape seedlings caused by low temperature.Key components of the response are:enhanced the activities of AI,NI,SS and SPS,increased soluble sugar level in leaves,promoting cold resistance ability for seedling.
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维普
