摘要
以青海省西宁市大通县鹞子沟自然发病的大刺儿菜叶片中分离出的附球属真菌DT-014为研究对象,通过发酵喷雾法接种测定其对4种盆栽杂草猪秧秧(Galium spurium L.)、藜(Chenopodium album L.)、冬葵(Malva ver-ticillate L.var.crispa)、密花香薷(Elsholtzia densa Benth.)的致病性和 5 种作物蚕豆(Vicia faba L.)、豌豆(Pisum sativum L.)、青稞(Hordeum vulgare L.)、小麦(Triticum aestivum L.)、油菜(Brassica napus L.)的安全性,并通过单因素试验,以菌落直径和其孢子悬浮液OD600值为测量标准,探究菌株DT-014的最适生长培养基、碳源、氮源、固态发酵基质.结果表明,DT-014对藜(C.album)和猪秧秧(G.spurium)致病力较强,其次为密花香薷(E.densa),对冬葵(M.crispa)致病力最低.DT-014 对于小麦(T.aestivum)、蚕豆(P.sativum)、青稞(H.vulgare)、豌豆(P.sativum)相对安全,对油菜(B.napus)有轻微影响.DT-014的最适培养基为蛋白肺培养基,最适碳源为葡萄糖,最适氮源为酵母膏,最适固态发酵基质为大豆粉.
Abstract
Fungus DT-014 of the Epicoccum,which was isolated from the leaves of Cephalanoplos setosum(Witld.)with naturally occurring disease in Yaozigou,Datong County,Xining City,Qinghai Province,was used as the research object.The pathogenicity of the strain DT-014 to 4 potted weeds Galium spurium L.,Chenopodium album L.,Malva verticillate L.var.Crispa,Elsholtzia densa Benth.and the safety of 5 potted crops Vicia faba L.,Pisum sativum L.,Hordeum vulgare L.,Triticum aestivum L.,Brassica napus L.were determined by fermentation spray inoculation.The single factor experiment method was used to explore the optimal growth medium,carbon source,nitrogen source and solid-state fermentation substrate of strain DT-014 by measuring the colony diameter and the OD600 value of spore suspension.The experimental results showed that DT-014 had strong pathogenicity to G.spurium and C.album,followed by E.densa and low pathogenicity to M.crispa.DT-014 was relatively safe for wheat T.aestivum,broad bean P.sativum,highland barley H.vulgare,and pea P.sativum,and slightly affected B.napus.The optimum medium for DT-014 was peptone medium,the optimum carbon source was glucose,the op-timum nitrogen source was yeast extract,and the optimum solid-state fermentation substrate was soybean powder.
基金项目
青海省科技厅国际合作专项(2023-HZ-808)
国家科技期刊平台
NSTL
维普
万方数据