Evaluation of salt tolerance of 80 introduced alfalfa varieties during germination
In this study,the effects of salt stress on root length,bud length,germination index,germination rate,germination energy,vitality index and root-bud ratio of 80 introduced alfalfa varieties treated with 1.2%NaCl were studied through laboratory germination test.A comprehensive evaluation of the salt tolerance of alfalfa during its germination period was conducted using a combination of principal component analysis,membership function,and cluster analysis.The results showed that salt stress significantly affected the germination energy,germination rate,and germination index of alfalfa(P<0.01),and the coefficient of variation between different varieties ranged from 0.008 to 0.723.The cumulative contribution rate of the two principal components was 73.922%.The germina-tion energy and root-bud ratio were used as the main salt tolerance indicators for salt tolerance screening during the germination period of alfalfa.The clustering analysis results divided 80 alfalfa varieties into 5 levels,including 9 va-rieties with strongest salt tolerance,14 varieties with strong salt tolerance,43 varieties with moderate salt tolerance,13 varieties with weak salt tolerance,and 1 variety with the weakest salt tolerance.On this basis,varieties of alfalfa with strong salt tolerance during the germination period were selected as follows:WL168HQ,Tourists,Tianma,Dite,Juneng 801,and Juneng 995.