摘要
通过室内萌发试验,以80份引进紫花苜蓿主推品种为材料,研究1.2％NaCl处理对紫花苜蓿萌发期根长、芽长、发芽指数、发芽率、发芽势、活力指数及根芽比的影响.采用主成分分析、隶属函数及聚类分析相结合的方法,对紫花苜蓿萌发期的耐盐性开展综合性评价.结果表明:盐胁迫极显著影响紫花苜蓿的发芽势、发芽率和发芽指数(P＜0.01),不同品种间的变异系数介于0.008～0.723.两个主成分的累计贡献率为73.922％.发芽势与根芽比可作为紫花苜蓿萌发期耐盐性筛选的主要评价指标.聚类分析结果将80份紫花苜蓿品种划分为5个级别,其中强耐盐级别包括9份品种;较强耐盐级别包括14份品种;中等耐盐级别包括43份品种;弱耐盐级别包括13份品种;最弱耐盐级别包括1份品种.在此基础上,筛选出萌发期强耐盐级别紫花苜蓿品种为:WL168HQ、游客、天马、迪特、巨能801和巨能995.
Abstract
In this study,the effects of salt stress on root length,bud length,germination index,germination rate,germination energy,vitality index and root-bud ratio of 80 introduced alfalfa varieties treated with 1.2％NaCl were studied through laboratory germination test.A comprehensive evaluation of the salt tolerance of alfalfa during its germination period was conducted using a combination of principal component analysis,membership function,and cluster analysis.The results showed that salt stress significantly affected the germination energy,germination rate,and germination index of alfalfa(P＜0.01),and the coefficient of variation between different varieties ranged from 0.008 to 0.723.The cumulative contribution rate of the two principal components was 73.922％.The germina-tion energy and root-bud ratio were used as the main salt tolerance indicators for salt tolerance screening during the germination period of alfalfa.The clustering analysis results divided 80 alfalfa varieties into 5 levels,including 9 va-rieties with strongest salt tolerance,14 varieties with strong salt tolerance,43 varieties with moderate salt tolerance,13 varieties with weak salt tolerance,and 1 variety with the weakest salt tolerance.On this basis,varieties of alfalfa with strong salt tolerance during the germination period were selected as follows:WL168HQ,Tourists,Tianma,Dite,Juneng 801,and Juneng 995.
基金项目
吉林省科技发展计划人才项目(20230508015RC)
现代农业产业技术体系(CARS-34)
维普
万方数据