Effects of biocontrol agents on fungal community diversity and structure in rhizosphere soil of continuous cropping celery
To provide a theoretical basis for alleviating the obstacles of continuous cropping of celery in the mountainous area of southern Ningxia,a field positioning experiment combined with high-flux sequencing technology was used to study the effects of conventional fertilization+mixed bactericides(MF),conventional ferti-lization+Bacillus subtilis(BS),and conventional fertilization+Trichoderma harzianum(TH)on fungal diversity and community composition of rhizosphere soil of celery in continuous cropping for 4 years,using conventional ferti-lization as a control(CK).The results showed that the application of Bacillus subtilis and Trichoderma harzianum compound bacteria increased the content of available phosphorus and available potassium in soil,with an increase of 34.29％and 9.98％,respectively.The application of microbial agents increased the activities of urease and su-crase,with an average increase of 43.16％and 12.98％,respectively,and significantly changed the β-diversity of soil fungi.Mortierella,Plectosphaerella,Gibberella,Alternaria,Fusarium were the dominant genera in each treat-ment.The application of compound microbial agent of Bacillus subtilis and Trichoderma harzianum treatment signifi-cantly reduced the relative abundance of fungal pathogens such as Gibberella,Alternaria and Fusarium by 59.88％,51.63％and 36.13％,respectively.Redundancy analysis showed that sucrase,urease,alkaline phosphatase,pH and total phosphorus were the main driving factors affecting the composition of the fungal community.In summary,the application of compound microbial agent of Bacillus subtilis and Trichoderma harzianum not only reduced the en-richment of pathogens such as Fusarium,Alternaria,and Gibberella,but also further reshaped the soil fungal com-munity structure by changing soil physical and chemical properties such as available phosphorus and potassium.
celerycontinuous croppingmicrobial agentsoil physical and chemical propertiesfungal com-munity structuremountainous area of southern Ningxia
万方数据
维普
