Effects of compound anti-salt agent on seed germination and growth of wheat under NaCl stress
Seven substances,including KNO3、CaCl2、DA-6、KH2PO4、FeSO4·7H2O、MgSO4·7H2O,and sucrose were used to formulate compound anti-salt agent.Using'Jimai 22'as test material,the water cultivaton method was employed to investigate the effects of different concentrations of anti-salt agent(10%,20%and 30%)on seed germination and subsequent seedling growth under different salt(NaCl)stress(0.2%,0.4%and 0.6%),and consequently the suitable formulation of anti-salt agent for wheat was proposed.The results showed that:(1)30%,20%and 10%anti-salt agent had the best effect in promoting wheat seed germination under 0.2%,0.4%and 0.6%salt stress,and the germination rate increased by 6.4%,12.0%and 26.5%,respectively.(2)Adding anti-salt a-gents at a salt concentration of 0.6%,root dry mass significantly increased by 29.7%to 35.9%.Photosynthetic rate was increased after the addition of anti-salt agent by 67.5%~90.0%(salt concentration 0.2%),13.3%~51.5%(salt concentration 0.4%)and 0.4%~25.5%(salt concentration 0.6%),respectively.The 30%anti-salt agent significantly enhanced photosynthesis at a salt concentration of 0.2%,with an increase of 90.0%.The photosynthetic pigment content of wheat was increased,with Chla content increased by 22.5%~39.0%(salt con-centration 0.2%),41.7%to 43.2%(salt concentration 0.4%);and Chlb content increased by 22.1%~31.0%(salt concentration 0.2%),14.7%~22.5%(salt concentration 0.4%);and carotenoid content increased by 12.2%to 28.6%(salt concentration 0.2%)and 24.4%to 32.3%(salt concentration 0.4%),respectively.In addi-tion,other physiological indicators also had corresponding improvement.(3)Based on the principal component a-nalysis,the results showed that wheat treated with 30%anti-salt agent had the highest score,so 30%anti-salt a-gent was the most suitable concentration ratio.