QTL mapping analysis of erucic acid content in rapeseed grains under drought conditions
To explore molecular genetic mechanism of the erucic acid accumulation in winter rapeseed grains under drought conditions,F,,F2 and F2∶3 populations derived from 17NTS57(high erucic acid)× CY12PXW-6-1(low erucic acid)were used as materials to analyze the genetic localization of erucic acid in this study.The re-sults showed that a total of 7 QTLs for erucic acid content were detected using the F2∶3 population in two condi-tions,including 4 QTLs for erucic acid content in Tianshui condition and 3 QTLs for erucic acid content in Dingxi condition.Two main QTLs were detected in the Tianshui condition,namely qEATC08.2 and qEATC09,distributed on chromosomes C08 and C09,with a phenotypic contribution rate of over 20%.qEATA10(Tianshui)and qEADA10(Dingxi)were in the same interval of A10 chromosome,and their phenotypic contributions in both environments were 10.42%and 12.70%,respectively.qEATC09 and qEADC09 were located in the same interval of the C09 chromosome,with phenotypic contribution rates of 20.0%and 19.34%in the two conditions,respectively,making them the main QTLs for co-localization.Three QTLs(qEATC08.1(Tianshui),qEATC08.2(Tianshui),qEADC07(Dingxi))were de-tected only in one condition,among which two minor modification sites(qEATC08.1 and qEADC07)had a positive additive effect.The major QTLs of qEA.A10 and qEA.C09 mapping on chromosomes A10 and C09 can be used for mo-lecular marker-assisted selection for the improvement of erucic acid content in rapeseed under drought conditions.