Amplification of glutathione reductase gene and its response to continuous cropping stress in soybean
The CDS of four GR genes were obtained in the soybean database Phytozome.The GR genes were cloned by RT-PCR using RNA from leaf and root as templates,respectively.The sizes of Glyma10g03740 and Gly-ma02g16010 were both 1 638 bp,indicating similar gene structures.The gene structures of Glyma16g27210 and Glyma02g08180 were similar with 1 506 bp.Phylogenetic analysis showed that Glyma10g03740/Glyma02g16010 and Glyma16g27210/Glyma02g08180 were clustered in different branches,while they were all closely related to Vigna un-guiculata,Phaseolus acutifolius and Phaseolus vulgaris,respectively.The four GRs had the same domains with FAD/NAD binding_dom and Pyr_Nucl dis_OxRdcase_Dimer.The tertiary structure showed that the conformations of Glyma16g27210 and Glyma02g08180 were similar,while those of Glyma10g03740 and Glyma02g16010 exhibited simi-larity as well.All four GRs existed in homodimers,with identical interacting proteins,including two thioredoxin re-ductases and eight thioredoxins.The RT-qPCR method was used to analyze the response of GRs to continuous crop-ping stress.The results showed that under continuous cropping stress,the GRs expression of sensitive variety HF55 was initiated earlier in roots,within 15 days after emergence,but it was activated later in leaves.The expression still showed an upward trend at 45 days after emergence.In the resistant variety KX8,the expression of GRs in roots and leaves reached their highest levels between 15 days and 45 days after emergence.At 30 days,the total expression of GRs in roots and leaves increased by 19.03 times and 2.50 times,respectively.
万方数据
维普
