Effects of biochar-earthworm in situ on rhizosphere microorganisms and fruit metabolism of facility tomatoes
To resolve the problems with excessive application of chemical fertilizers,resulting in poor quality and yield of facility tomatoes,different soil improvement measures were adopted.The experiment used'Fenyan No.1'tomato as the test material,and included blank control(CK),biochar(B),earthworm in situ(V)and biochar+earthworm in situ(BV)to study the changes in the physical and chemical properties of the root zone soil and soil rhizosphere microorganisms during the growth and development of tomatoes as well as the differences in fruit yield and quality during the fruiting period of tomatoes.The differences in metabolite composition of fruits un-der different treatments were analyzed.The results showed that BV treatment significantly increased soil available nitrogen,available phosphorus,and available potassium contents when compared with CK by 174％,7.44％,and 45％respectively.BV treatment significantly increased sucrase activity by 62.02％and V treatment significantly in-creased urease by 39.72％compared with CK.BV treatment significantly increased the Simpson index of soil bacte-ria,and V treatment significantly increased the Ace and Chaol index of soil fungi.B treatment significantly changed the relative abundance of soil bacteria,with Proteobacteria increasing the most,reaching 45.48％,Ac-idobacteria and The Bacillus Monospora phylum decreased the mostby 14.90％and 25.70％respectively.For soil fungi,the relative abundance of Ascomycota in treatment B was the highest,increasing by 15.90％,and the V treatment had the highest relative abundance of Oychytrids with 7.24％and Rhododendron increased second,reac-hing 3.11％.At the same time,each treatment significantly improved fruit yield and quality.BV treatment had the best tomato yield and quality effects,as shown by its highest biomass,fruit yield,soluble solids,Vc,and organic acid content.Based on the analysis of the fruit differential metabolites,the tomato fruits obtained in the V treatment had the largest variety of differential metabolites,the greatest difference,and the largest number of differential met-abolic pathwayscompared with the control group.
万方数据
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